{"code":"SASDXG2","status":"Published","type_of_curve":"Single concentration","angular_unit":"1/A","project":{"title":"Insight into the structure and interactions of the <i>M. tuberculosis</i>\n            Mce-associated membrane proteins Mam1A-1D","publication":{"title":"Insight into the structure and interactions of the <i>M. tuberculosis</i>\n            Mce-associated membrane proteins Mam1A-1D","author_list":"Hynönen M, Perumal P, Hynönen N, Doutch J, Ma K, Venkatesan R","journal":null,"doi":"10.1101/2025.03.26.645568","pmid":null,"published_date":"2025 Mar 26"},"status":"released","submitted_date":"2025-03-26","released_date":"2025-04-11"},"pddf_data":"https://www.sasbdb.org/media/p_of_R_files/SASDXG2.out","intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDXG2.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDXG2_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDXG2_kratky_img.png","pddf_plot":"https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDXG2_pofr_img.png","intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDXG2_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDXG2.sascif","experiment":{"instrument":{"detector":null,"name":"ISIS Neutron and Muon Source","city":"Didcot","country":"UK","beamline_name":"SANS2D","beam_geometry":null,"type_of_source":"neutron source","point_source":null,"line_collimation":null,"sample_path_length":null,"line_collimation_slitlength":null,"line_collimation_integrationwidth":null,"xray_energy":null,"beam_profile_ah":null,"beam_profile_al":null},"sample":{"molecule":[{"long_name":"Probable conserved Mce associated membrane protein","short_name":"Mam1A delta 106","sequence":"MKHHHHHHPMSDYDIPTTENLYFQGAMAPDAGAMSASMQKIIECGTGDFGAQASLYTSMLVEAYQAASVHVQVTDMRAAVERNNNDGSVDVLVALRVKVSNTDSDAHEVGYRLRVRMALDEGRYKIAKLDQVTK","organism":"Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)","uniprot_code":"O07419","uniprot_range_first":107,"uniprot_range_last":213,"oligomerization":"tetramer","molecular_type":"protein","uniprot_sequence":"MKAADSAESDAGADQTGPQVKAADSAESDAGELGEDACPEQALVERRPSRLRRGWLVGIA\nATLLALAGGLGAAGYFALRSHQESQSIAREDLAAIEAAKDCVAATQAPDAGAMSASMQKI\nIECGTGDFGAQASLYTSMLVEAYQAASVHVQVTDMRAAVERNNNDGSVDVLVALRVKVSN\nTDSDAHEVGYRLRVRMALDEGRYKIAKLDQVTK","mw":14.807,"total_mw":59.228,"number_molecules":4,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":"Mam1A/Rv0175 delta 106 is N-terminally truncated construct which lacks the alpha1-helix. Protein, despite the removal of TM-helix, is not soluble and required C12E9 detergent to be solubilized. Tetrameric form was calculated with SEC-MALS."}],"buffer":{"name":"50 mM Tris, 500 mM NaCl, D-C12E9","concentration_unit":null,"comment":"50mM Tris at pH8.5 with 500mM NaCl, supplemented with 0.003% Deuterated C12E9","additive":null,"concentration":null,"pka":null,"ph":8.5,"deuteration":null},"purity_method":null,"name":"Probable conserved Mce associated membrane protein (Mam1Adelta106) from M.tuberculosis (SANS)","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[{"affiliation":[{"short_name":null,"address":null,"full_name":"University of Helsinki","webpage":null}],"contributor_name":"Mikko","contributor_surname":"Hynönen","orcid":"https://orcid.org/0000-0003-2431-8892"},{"affiliation":[{"short_name":null,"address":"Finland","full_name":"University of Oulu","webpage":"https://www.oulu.fi"}],"contributor_name":"Rajaram","contributor_surname":"Venkatesan","orcid":"https://orcid.org/0000-0002-7257-1211"}],"concentration_method":null,"concentration_unit":null,"date":"2024-04-25","storage_temperature":4.0,"cell_temperature":6.0,"exposure_time":null,"number_of_frames":null,"wavelength":null,"sample_detector_distance":null,"concentration_min":null,"concentration_max":null,"sample_volume":35.0,"flow_rate":0.07,"s_min":0.062,"s_max":7.827,"total_exposure_time":null,"seccolumn":null},"fits":[{"models":[{"model_plot":"https://www.sasbdb.org/media/pdb_file/images/1A_107_Main_Model_Tetra_img.png","software":"AlphaFold","pdb_link":[],"model_title":"1A_107_Main_Model_Tetra_SASDXG2","type_of_model":"atomic","software_version":null,"model_data":"https://www.sasbdb.org/media/pdb_file/1A_107_Main_Model_Tetra.pdb","model_mw":59.49,"bead_radius":1.9,"log":null,"symmetry":null,"comment":null,"user":1328}],"fit_unit":"1/A","fit_plot":"https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDXG2_fit1_fixed_fit_img.png","software":"CRYSON","chi_square_value":2.58,"p_value":0.0272,"fit_residual_plot":"SASDXG2_fit1_fitresiduals_img.png","fit_data":"https://www.sasbdb.org/media/fitting_files/SASDXG2_fit1.fit","fit_log":"https://www.sasbdb.org/media/fitting_files/log_files/SASDXG2_1A_107_Main_Model_Cryson_3Bcycig.log","software_version":null,"description":""}],"estimated_volume_method":null,"pddf_software":"ATSAS GNOM","pddf_software_version":"5.0","i0_calibration_standard":null,"description":"SANS data were collected on the Zoom time-of-flight SANS instrument (ISIS Neutron and Muon Source, Harwell, UK) using wavelength range 1.75-16.5Å. The source to sample and sample to detector distance was 4 m and the beam size was 12 mm. Mam1Adelta106 was solubilized with C12E9 in the lysis buffer where the deuteration of the C12E9 was 0.2 % v/v. Excess C12E9 was washed away throughout the purification, and replaced with D-C12E9 in the STFC SANS facilities using SEC Superdex200 column.  From the highest point of the peak, 3 fractions were selected. Each of these fractions was loaded to Starna Scientific quartz round cuvette (match code 6,path length 1, type 32/Q/1), and SANS data were collected from each fraction with an exposure time of 1 h in 5 cycles (5 h total exposure time for each fraction). The neutron momentum transfer (q) standard deviation and truncated scattering data are provided as an additional file.","experiment_description":null,"tags":[],"intensity_unit":"arbitrary","experimental_mw":33.1,"experimental_mw_error":null,"guinier_i0_mw":null,"guinier_i0_mw_error":null,"porod_mw":20.0,"porod_mw_error":null,"pddf_i0":0.126,"pddf_i0_error":null,"guinier_i0":0.124085,"guinier_i0_error":null,"pddf_rg":2.58,"pddf_rg_error":null,"guinier_rg":2.553,"guinier_rg_error":0.683,"pddf_dmax":8.5,"pddf_dmax_error":null,"porod_volume":70.0,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":17,"guinier_point_last":28,"pddf_point_first":18,"pddf_point_last":44,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDXG2_datloglog_img.png","symmetry":null,"last_modified":"2026-05-05T11:41:40.417306+02:00","bragg_peak":[]}