{"code":"SASDXL8","status":"Published","type_of_curve":"SEC-SAS","angular_unit":"1/A","project":{"title":"From TDP-43/RNA complex formation to disease-linked TDP-43 aggregation through a structural and cellular approach.","publication":{"title":"From TDP-43/RNA complex formation to disease-linked TDP-43 aggregation through a structural and cellular approach.","author_list":"Feng Y, Joshi V, Pankivskyi S, Clément MJ, Rengifo-Gonzalez JC, Thureau A, Pastré D, Bouhss A","journal":"Nat Commun","doi":"10.1038/s41467-026-68346-y","pmid":"41565639","published_date":"2026 Jan 21"},"status":"released","submitted_date":"2025-09-08","released_date":"2026-01-25"},"pddf_data":"https://www.sasbdb.org/media/p_of_R_files/SASDXL8.out","intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDXL8.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDXL8_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDXL8_kratky_img.png","pddf_plot":"https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDXL8_pofr_img.png","intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDXL8_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDXL8.sascif","experiment":{"instrument":{"detector":{"type":null,"name":"Eiger 4M","resolution":75.0},"name":"SOLEIL","city":"Saint-Aubin","country":"France","beamline_name":"SWING","beam_geometry":null,"type_of_source":"X-ray synchrotron","point_source":null,"line_collimation":null,"sample_path_length":null,"line_collimation_slitlength":null,"line_collimation_integrationwidth":null,"xray_energy":null,"beam_profile_ah":null,"beam_profile_al":null},"sample":{"molecule":[{"long_name":"TAR DNA-binding protein 43","short_name":"TDP-43 - WT","sequence":"MSEYIRVTEDENDEPIEIPSEDDGTVLLSTVTAQFPGACGLRYRNPVSQCMRGVRLVEGILHAPDAGWGNLVYVVNYPKDNKRKMDETDASSAVKVKRAVQKTSDLIVLGLPWKTTEQDLKEYFSTFGEVLMVQVKKDLKTGHSKGFGFVRFTEYETQVKVMSQRHMIDGRWCDCKLPNSKQSQDEPLRSRKVFVGRCTEDMTEDELREFFSQYGDVMDVFIPKPFRAFAFVTFADDQIAQSLCGEDLIIKGISVHISNAEPKHNSNRQLERSGRFG","organism":"Homo sapiens","uniprot_code":"Q13148","uniprot_range_first":1,"uniprot_range_last":277,"oligomerization":"monomer","molecular_type":"protein","uniprot_sequence":"MSEYIRVTEDENDEPIEIPSEDDGTVLLSTVTAQFPGACGLRYRNPVSQCMRGVRLVEGI\nLHAPDAGWGNLVYVVNYPKDNKRKMDETDASSAVKVKRAVQKTSDLIVLGLPWKTTEQDL\nKEYFSTFGEVLMVQVKKDLKTGHSKGFGFVRFTEYETQVKVMSQRHMIDGRWCDCKLPNS\nKQSQDEPLRSRKVFVGRCTEDMTEDELREFFSQYGDVMDVFIPKPFRAFAFVTFADDQIA\nQSLCGEDLIIKGISVHISNAEPKHNSNRQLERSGRFGGNPGGFGNQGGFGNSRGGGAGLG\nNNQGSNMGGGMNFGAFSINPAMMAAAQAALQSSWGMMGMLASQQNQSGPSGNNQNQGNMQ\nREPNQAFGSGNNSYSGSNSGAAIGWGSASNAGSGSGFNGGFGSSMDSKSSGWGM","mw":31.5,"total_mw":63.0,"number_molecules":2,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":"RNA-binding protein that is involved in various steps of RNA biogenesis and processing.\r\nCo-exist in a mixure of monomere and dimer"}],"buffer":{"name":"20 mM HEPES, 100 mM KCl, 2 mM TCEP","concentration_unit":null,"comment":null,"additive":null,"concentration":null,"pka":null,"ph":7.6,"deuteration":null},"purity_method":null,"name":"A nuclear mRNA-binding protein bound to GU-repeat RNA Oligo: TDP-43 (TAR RNA-binding protein 43)","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[{"affiliation":[{"short_name":"Soleil","address":"Saint-Aubin, France","full_name":"Soleil Synchrotron","webpage":"http://www.synchrotron-soleil.fr/"}],"contributor_name":"Aurélien","contributor_surname":"Thureau","orcid":null}],"concentration_method":null,"concentration_unit":null,"date":"2022-11-30","storage_temperature":10.0,"cell_temperature":25.0,"exposure_time":1.0,"number_of_frames":600,"wavelength":0.1033,"sample_detector_distance":2.0,"concentration_min":null,"concentration_max":1.0,"sample_volume":50.0,"flow_rate":0.3,"s_min":0.059,"s_max":5.516,"total_exposure_time":null,"seccolumn":34},"fits":[],"estimated_volume_method":null,"pddf_software":"ATSAS GNOM","pddf_software_version":null,"i0_calibration_standard":null,"description":"15 µL of ssRNA (GU)12 was incubated with 50 µL of TDP-43 during 1 hour at room temperature to reach a final ratio for the complex TDP-43:ssRNA of 2:1 with 60 µM of TDP-43 and 27 µM of ssRNA. 50 µL of this complex was injected into the SEC column.","experiment_description":null,"tags":[],"intensity_unit":"arbitrary","experimental_mw":87.6,"experimental_mw_error":null,"guinier_i0_mw":null,"guinier_i0_mw_error":null,"porod_mw":84.0,"porod_mw_error":null,"pddf_i0":0.01252,"pddf_i0_error":null,"guinier_i0":0.01242,"guinier_i0_error":null,"pddf_rg":4.11,"pddf_rg_error":null,"guinier_rg":3.98,"guinier_rg_error":0.261,"pddf_dmax":16.5,"pddf_dmax_error":null,"porod_volume":134.5,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":23,"guinier_point_last":50,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDXL8_datloglog_img.png","symmetry":null,"last_modified":"2026-09-03T10:12:36.770206+02:00","bragg_peak":[]}