{"code":"SASDXQ5","status":"Published","type_of_curve":"Single concentration","angular_unit":"1/A","project":{"title":"A domain-swapped CaMKII conformation facilitates linker-mediated allosteric regulation - revision","publication":null,"status":"released","submitted_date":"2025-07-01","released_date":"2025-09-03"},"pddf_data":null,"intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDXQ5.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDXQ5_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDXQ5_kratky_img.png","pddf_plot":null,"intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDXQ5_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDXQ5.sascif","experiment":{"instrument":{"detector":{"type":"Reverse-biased silicon diode array","name":"DECTRIS/ PILATUS 100K","resolution":172.0},"name":"Institute for Applied Life Sciences/Biophysical Characterization","city":"Amherst","country":"United States","beamline_name":"Rigaku/BioSAXS-2000 SAXS","beam_geometry":null,"type_of_source":"X-ray in house","point_source":null,"line_collimation":null,"sample_path_length":null,"line_collimation_slitlength":null,"line_collimation_integrationwidth":null,"xray_energy":null,"beam_profile_ah":null,"beam_profile_al":null},"sample":{"molecule":[{"long_name":"Calcium/calmodulin-dependent protein kinase type II subunit alpha (Δ1-6; Q223K; Δ 316-329)","short_name":"CaMKII Exon 18","sequence":"TRFTEEYQLFEELGKGAFSVVRRCVKVLAGQEYAAKIINTKKLSARDHQKLEREARICRLLKHPNIVRLHDSISEEGHHYLIFDLVTGGELFEDIVAREYYSEADASHCIQQILEAVLHCHQMGVVHRDLKPENLLLASKLKGAAVKLADFGLAIEVEGEQQAWFGFAGTPGYLSPEVLRKDPYGKPVDLWACGVILYILLVGYPPFWDEDQHRLYKQIKAGAYDFPSPEWDTVTPEAKDLINKMLTINPSKRITAAEALKHPWISHRSTVASCMHRQETVDCLKKFNARRKLKGAILTTMLATRNFSESSESTNTTIEDEDTKVRKQEIIKVTEQLIEAISNGDFESYTKMCDPGMTAFEPEALGNLVEGLDFHRFYFENLWSRNSKPVHTTILNPHIHLMGDESACIAYIRITQYLDAGGIPRTAQSEETRVWHRRDGKWQIVHFHRSGAPSVLPH","organism":"Homo sapiens","uniprot_code":"Q9UQM7","uniprot_range_first":7,"uniprot_range_last":478,"oligomerization":"dodecamer","molecular_type":"protein","uniprot_sequence":"MATITCTRFTEEYQLFEELGKGAFSVVRRCVKVLAGQEYAAKIINTKKLSARDHQKLERE\nARICRLLKHPNIVRLHDSISEEGHHYLIFDLVTGGELFEDIVAREYYSEADASHCIQQIL\nEAVLHCHQMGVVHRDLKPENLLLASKLKGAAVKLADFGLAIEVEGEQQAWFGFAGTPGYL\nSPEVLRKDPYGKPVDLWACGVILYILLVGYPPFWDEDQHRLYQQIKAGAYDFPSPEWDTV\nTPEAKDLINKMLTINPSKRITAAEALKHPWISHRSTVASCMHRQETVDCLKKFNARRKLK\nGAILTTMLATRNFSGGKSGGNKKSDGVKESSESTNTTIEDEDTKVRKQEIIKVTEQLIEA\nISNGDFESYTKMCDPGMTAFEPEALGNLVEGLDFHRFYFENLWSRNSKPVHTTILNPHIH\nLMGDESACIAYIRITQYLDAGGIPRTAQSEETRVWHRRDGKWQIVHFHRSGAPSVLPH","mw":52.166,"total_mw":625.992,"number_molecules":12,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":"The linker region only contains exon 18."}],"buffer":{"name":"50 mM Tris , 0.2 mM CaCl2, 150 mM KCl, 10 mM MgCl2, 2 mM ATP","concentration_unit":null,"comment":"no caM","additive":null,"concentration":null,"pka":null,"ph":8.0,"deuteration":null},"purity_method":null,"name":"Calcium/calmodulin-dependent protein kinase type II (CaMKII) subunit holoenzyme with Exon 18 as the linker region + 100 nM Ca2+/CaM","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[{"affiliation":[{"short_name":null,"address":null,"full_name":"university of massachusetts amherst","webpage":null}],"contributor_name":"Bao","contributor_surname":"Nguyen Viet","orcid":""}],"concentration_method":null,"concentration_unit":null,"date":"2024-12-18","storage_temperature":10.0,"cell_temperature":22.0,"exposure_time":600.0,"number_of_frames":1,"wavelength":0.154,"sample_detector_distance":0.4389,"concentration_min":null,"concentration_max":1.5,"sample_volume":null,"flow_rate":null,"s_min":0.066,"s_max":6.962,"total_exposure_time":null,"seccolumn":null},"fits":[],"estimated_volume_method":null,"pddf_software":null,"pddf_software_version":null,"i0_calibration_standard":null,"description":null,"experiment_description":null,"tags":[],"intensity_unit":"arbitrary","experimental_mw":712.3,"experimental_mw_error":null,"guinier_i0_mw":null,"guinier_i0_mw_error":null,"porod_mw":null,"porod_mw_error":null,"pddf_i0":null,"pddf_i0_error":null,"guinier_i0":3.32195,"guinier_i0_error":null,"pddf_rg":null,"pddf_rg_error":null,"guinier_rg":7.205,"guinier_rg_error":1.942,"pddf_dmax":null,"pddf_dmax_error":null,"porod_volume":null,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":3,"guinier_point_last":9,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDXQ5_datloglog_img.png","symmetry":null,"last_modified":"2025-09-03T03:50:46.457487+02:00","bragg_peak":[]}