{"code":"SASDXX5","status":"Published","type_of_curve":"Single concentration","angular_unit":"1/A","project":{"title":"Phosphorylation toggles the SARS-CoV-2 nucleocapsid protein between two membrane-associated condensate states.","publication":{"title":"Phosphorylation toggles the SARS-CoV-2 nucleocapsid protein between two membrane-associated condensate states.","author_list":"Favetta B, Wang H, Cubuk J, Singh A, Barai M, Ramirez C, Zheng H, Gormley AJ, Murthy NS, Dignon G, Soranno A, Shi Z, Schuster BS","journal":"Nat Commun","doi":"10.1038/s41467-025-62922-4","pmid":"40858555","published_date":"2025 Aug 26"},"status":"released","submitted_date":"2025-07-03","released_date":"2025-08-19"},"pddf_data":null,"intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDXX5.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDXX5_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDXX5_kratky_img.png","pddf_plot":null,"intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDXX5_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDXX5.sascif","experiment":{"instrument":{"detector":{"type":"Duel detector","name":"Pilatus3 S 1M, Pilatus3 900 K","resolution":null},"name":"National Synchrotron Light Source II (NSLS-II)","city":"Upton, NY","country":"USA","beamline_name":"16-ID (LiX)","beam_geometry":null,"type_of_source":"X-ray synchrotron","point_source":true,"line_collimation":null,"sample_path_length":null,"line_collimation_slitlength":null,"line_collimation_integrationwidth":null,"xray_energy":null,"beam_profile_ah":null,"beam_profile_al":null},"sample":{"molecule":[{"long_name":"Nucleoprotein","short_name":null,"sequence":"MHHHHHHENLYFQGSDNGPQNQRNAPRITFGGPSDSTGSNQNGERSGARSKQRRPQGLPNNTASWFTALTQHGKEDLKFPRGQGVPINTNSSPDDQIGYYRRATRRIRGGDGKMKDLSPRWYFYYLGTGPEAGLPYGANKDGIIWVATEGALNTPKDHIGTRNPANNAAIVLQLPQGTTLPKGFYAEGSRGGSQASSRSSSRSRNSSRNSTPGSSRGTSPARMAGNGGDAALALLLLDRLNQLESKMSGKGQQQQGQTVTKKSAAEASKKPRQKRTATKAYNVTQAFGRRGPEQTQGNFGDQELIRQGTDYKHWPQIAQFAPSASAFFGMSRIGMEVTPSGTWLTYTGAIKLDDKDPNFKDQVILLNKHIDAYKTFPPTEPKKDKKKKADETQALPQRQKKQQTVTLLPAADLDDFSKQLQQSMSSADSTQA","organism":"Severe acute respiratory syndrome coronavirus 2","uniprot_code":"P0DTC9","uniprot_range_first":2,"uniprot_range_last":419,"oligomerization":"dimer","molecular_type":"protein","uniprot_sequence":"MSDNGPQNQRNAPRITFGGPSDSTGSNQNGERSGARSKQRRPQGLPNNTASWFTALTQHG\nKEDLKFPRGQGVPINTNSSPDDQIGYYRRATRRIRGGDGKMKDLSPRWYFYYLGTGPEAG\nLPYGANKDGIIWVATEGALNTPKDHIGTRNPANNAAIVLQLPQGTTLPKGFYAEGSRGGS\nQASSRSSSRSRNSSRNSTPGSSRGTSPARMAGNGGDAALALLLLDRLNQLESKMSGKGQQ\nQQGQTVTKKSAAEASKKPRQKRTATKAYNVTQAFGRRGPEQTQGNFGDQELIRQGTDYKH\nWPQIAQFAPSASAFFGMSRIGMEVTPSGTWLTYTGAIKLDDKDPNFKDQVILLNKHIDAY\nKTFPPTEPKKDKKKKADETQALPQRQKKQQTVTLLPAADLDDFSKQLQQSMSSADSTQA","mw":47.3,"total_mw":94.6,"number_molecules":2,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":"The protein construct contains an additional 14 non-native amino acids at the N-terminus including a polyhistidine tag (MHHHHHHENLYFQG).\r\nN-terminal His6xtag+tev cleavage site"}],"buffer":{"name":"20 mM Tris, 150 mM NaCl","concentration_unit":null,"comment":null,"additive":null,"concentration":null,"pka":null,"ph":7.5,"deuteration":null},"purity_method":null,"name":"SARS-CoV-2 Nucleocapsid protein 0.5 mg/mL","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[{"affiliation":[{"short_name":null,"address":null,"full_name":"Rutgers University","webpage":null}],"contributor_name":"Bruna","contributor_surname":"Favetta","orcid":"https://orcid.org/0000-0002-3613-1665"}],"concentration_method":null,"concentration_unit":null,"date":"2023-07-18","storage_temperature":4.0,"cell_temperature":25.0,"exposure_time":0.5,"number_of_frames":10,"wavelength":0.08189,"sample_detector_distance":3.56,"concentration_min":null,"concentration_max":0.5,"sample_volume":null,"flow_rate":null,"s_min":0.05,"s_max":2.5,"total_exposure_time":null,"seccolumn":null},"fits":[],"estimated_volume_method":null,"pddf_software":"BioXTAS RAW","pddf_software_version":null,"i0_calibration_standard":null,"description":null,"experiment_description":"Synchrotron SAXS data from solutions of SARS-CoV-2 Nucleocapsid protein in 20 mM Tris, 150 mM NaCl, pH 7.5 were collected on the 16-ID (LiX) beam line at the National Synchrotron Light Source II (NSLS-II) storage ring (Upton, NY, USA)  of λ = 0.08189 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). SAXS data was collected over a s range of 0.05 - 31.3 nm-1 and analyzed within a range of 0.05 - 2.55 nm-1. Sample to detector distance for small angles was 3.560 m and 0.317 m for wide angles using Pilatus3X 1M and Pilatus3X 900k detectors. One solute concentration of 0.50 mg/ml was measured at 25°C. 10 successive 0.500 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.","tags":[],"intensity_unit":"arbitrary","experimental_mw":94.6,"experimental_mw_error":null,"guinier_i0_mw":null,"guinier_i0_mw_error":null,"porod_mw":null,"porod_mw_error":null,"pddf_i0":1.1415,"pddf_i0_error":0.0023,"guinier_i0":1.44685,"guinier_i0_error":null,"pddf_rg":5.713,"pddf_rg_error":0.11,"guinier_rg":5.768,"guinier_rg_error":0.17,"pddf_dmax":17.05,"pddf_dmax_error":0.99,"porod_volume":null,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":1,"guinier_point_last":19,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDXX5_datloglog_img.png","symmetry":null,"last_modified":"2025-09-01T15:11:14.414737+02:00","bragg_peak":[]}