{"code":"SASDXX7","status":"Published","type_of_curve":"SEC-SAS","angular_unit":"1/nm","project":{"title":"The N-Myc MB0-MBI region interacts specifically and dynamically with the N-lobe of Aurora kinase A.","publication":{"title":"The N-Myc MB0-MBI region interacts specifically and dynamically with the N-lobe of Aurora kinase A.","author_list":"Hultman J, Morad V, Tanner E, Kenney TMG, Pietras Z, Khare LP, Derbyshire D, Resetca D, Arrowsmith CH, Aili D, Ekström S, Penn LZ, Wallner B, Ahlner A, Sunnerhagen M","journal":"Nat Commun","doi":"10.1038/s41467-026-69725-1","pmid":"41735282","published_date":"2026 Feb 24"},"status":"released","submitted_date":"2025-08-19","released_date":"2026-01-30"},"pddf_data":"https://www.sasbdb.org/media/p_of_R_files/SASDXX7.out","intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDXX7.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDXX7_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDXX7_kratky_img.png","pddf_plot":"https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDXX7_pofr_img.png","intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDXX7_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDXX7.sascif","experiment":{"instrument":{"detector":{"type":null,"name":"Pilatus 6M","resolution":null},"name":"PETRA III","city":"DESY; Hamburg","country":"Germany","beamline_name":"EMBL P12","beam_geometry":null,"type_of_source":"X-ray synchrotron","point_source":null,"line_collimation":null,"sample_path_length":null,"line_collimation_slitlength":null,"line_collimation_integrationwidth":null,"xray_energy":null,"beam_profile_ah":null,"beam_profile_al":null},"sample":{"molecule":[{"long_name":"N-myc proto-oncogene protein, residues 1-69","short_name":"N-Myc1-69","sequence":"SMPSCSTSTMP GMICKNPDLE FDSLQPCFYP DEDDFYFGGP DSTPPGEDIW KKFELLPTPP\r\nLSPSRGFAE","organism":"Homo sapiens","uniprot_code":"P04198","uniprot_range_first":1,"uniprot_range_last":69,"oligomerization":"monomer","molecular_type":"protein","uniprot_sequence":"MPSCSTSTMPGMICKNPDLEFDSLQPCFYPDEDDFYFGGPDSTPPGEDIWKKFELLPTPP\nLSPSRGFAEHSSEPPSWVTEMLLENELWGSPAEEDAFGLGGLGGLTPNPVILQDCMWSGF\nSAREKLERAVSEKLQHGRGPPTAGSTAQSPGAGAASPAGRGHGGAAGAGRAGAALPAELA\nHPAAECVDPAVVFPFPVNKREPAPVPAAPASAPAAGPAVASGAGIAAPAGAPGVAPPRPG\nGRQTSGGDHKALSTSGEDTLSDSDDEDDEEEDEEEEIDVVTVEKRRSSSNTKAVTTFTIT\nVRPKNAALGPGRAQSSELILKRCLPIHQQHNYAAPSPYVESEDAPPQKKIKSEASPRPLK\nSVIPPKAKSLSPRNSDSEDSERRRNHNILERQRRNDLRSSFLTLRDHVPELVKNEKAAKV\nVILKKATEYVHSLQAEEHQLLLEKEKLQARQQQLLKKIEHARTC","mw":7.766,"total_mw":7.766,"number_molecules":1,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":""},{"long_name":"Aurora kinase A mutant C290A:C393A","short_name":"AurA C290A:C393A","sequence":"ESKKRQWALE DFEIGRPLGK GKFGNVYLAR EKQSKFILAL KVLFKAQLEK AGVEHQLRRE\r\nVEIQSHLRHP NILRLYGYFH DATRVYLILE YAPLGTVYRE LQKLSKFDEQ RTATYITELA\r\nNALSYCHSKR VIHRDIKPEN LLLGSAGELK IADFGWSVHA PSSRRTTLAG TLDYLPPEMI\r\nEGRMHDEKVD LWSLGVLCYE FLVGKPPFEA NTYQETYKRI SRVEFTFPDF VTEGARDLIS\r\nRLLKHNPSQR PMLREVLEHP WITANSSKPS NAQNKESASK QS","organism":"Homo sapiens","uniprot_code":"O14965","uniprot_range_first":122,"uniprot_range_last":403,"oligomerization":"monomer","molecular_type":"protein","uniprot_sequence":"MDRSKENCISGPVKATAPVGGPKRVLVTQQFPCQNPLPVNSGQAQRVLCPSNSSQRIPLQ\nAQKLVSSHKPVQNQKQKQLQATSVPHPVSRPLNNTQKSKQPLPSAPENNPEEELASKQKN\nEESKKRQWALEDFEIGRPLGKGKFGNVYLAREKQSKFILALKVLFKAQLEKAGVEHQLRR\nEVEIQSHLRHPNILRLYGYFHDATRVYLILEYAPLGTVYRELQKLSKFDEQRTATYITEL\nANALSYCHSKRVIHRDIKPENLLLGSAGELKIADFGWSVHAPSSRRTTLCGTLDYLPPEM\nIEGRMHDEKVDLWSLGVLCYEFLVGKPPFEANTYQETYKRISRVEFTFPDFVTEGARDLI\nSRLLKHNPSQRPMLREVLEHPWITANSSKPSNCQNKESASKQS","mw":32.573,"total_mw":32.573,"number_molecules":1,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":""}],"buffer":{"name":"20mM HEPES, 150mM NaCl, 5mM MgCl2, 3% v/v glycerol, 2mM TCEP","concentration_unit":null,"comment":null,"additive":null,"concentration":null,"pka":null,"ph":7.5,"deuteration":null},"purity_method":null,"name":"N-Myc proto-oncogene residues 1-69 in complex with Aurora kinase A mutant C290A:C393A","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[{"affiliation":[{"short_name":null,"address":null,"full_name":"Linköping University ","webpage":null}],"contributor_name":"Johanna","contributor_surname":"Hultman","orcid":"https://orcid.org/0009-0000-8066-228X"}],"concentration_method":null,"concentration_unit":null,"date":"2023-11-28","storage_temperature":null,"cell_temperature":null,"exposure_time":null,"number_of_frames":null,"wavelength":null,"sample_detector_distance":null,"concentration_min":null,"concentration_max":null,"sample_volume":null,"flow_rate":null,"s_min":0.023,"s_max":4.461,"total_exposure_time":null,"seccolumn":null},"fits":[],"estimated_volume_method":null,"pddf_software":"ATSAS GNOM","pddf_software_version":"5.0","i0_calibration_standard":null,"description":"Synchrotron SAXS data from solutions of the N-Myc proto-oncogen protein (residues 1-69) in complex with the kinase domain of Aurora kinase A (residues 122-403, containing a C290A:C393A double mutation) was collected in 20mM HEPES, 150mM NaCl, 5mM MgCl2, 3% v/v glycerol, 2mM TCEP, pH 7.5 on the EMBL P12 beam line at PETRA III (DESY, Hamburg, Germany) using a Pilatus 6M detector at a sample-detector distance of 3 m and a wavelength of λ = 0.124 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAXS was employed by injecting 90.00 μl sample of 10 mg/ml onto a GE Superdex 75 Increase 10/300 column with a flow rate of 0.70 ml/min at 20°C. 2100 successive 1 second frames were collected. Buffer subtraction was done using CHROMIXS and data analysis was done using the ATSAS suite.","experiment_description":null,"tags":[],"intensity_unit":"arbitrary","experimental_mw":45.3,"experimental_mw_error":null,"guinier_i0_mw":null,"guinier_i0_mw_error":null,"porod_mw":38.0,"porod_mw_error":null,"pddf_i0":2240.0,"pddf_i0_error":null,"guinier_i0":2224.8,"guinier_i0_error":null,"pddf_rg":2.537,"pddf_rg_error":null,"guinier_rg":2.47,"guinier_rg_error":0.007,"pddf_dmax":10.2,"pddf_dmax_error":null,"porod_volume":62.09,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":70,"guinier_point_last":174,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDXX7_datloglog_img.png","symmetry":null,"last_modified":"2026-02-25T14:53:49.153089+01:00","bragg_peak":[]}