{"code":"SASDXX8","status":"Published","type_of_curve":"Single concentration","angular_unit":"1/A","project":{"title":"Ion-selective conformational stabilization of a disordered repeats-in-toxin protein domain.","publication":{"title":"Ion-selective conformational stabilization of a disordered repeats-in-toxin protein domain.","author_list":"Gudinas AP, Shambharkar GM, Chang MP, Fernández D, Matsui T, Mai DJ","journal":"Biophys J","doi":"10.1016/j.bpj.2025.10.014","pmid":"41088755","published_date":"2025 Oct 13"},"status":"released","submitted_date":"2025-09-05","released_date":"2025-10-16"},"pddf_data":"https://www.sasbdb.org/media/p_of_R_files/SASDXX8.out","intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDXX8.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDXX8_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDXX8_kratky_img.png","pddf_plot":"https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDXX8_pofr_img.png","intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDXX8_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDXX8.sascif","experiment":{"instrument":{"detector":{"type":null,"name":"Pilatus3 X 1M","resolution":0.172},"name":"Stanford Synchrotron Radiation Lightsource (SSRL)","city":"Menlo Park, CA","country":"USA","beamline_name":"BL4-2","beam_geometry":null,"type_of_source":"X-ray synchrotron","point_source":null,"line_collimation":null,"sample_path_length":null,"line_collimation_slitlength":null,"line_collimation_integrationwidth":null,"xray_energy":null,"beam_profile_ah":null,"beam_profile_al":null},"sample":{"molecule":[{"long_name":"Repeats-in-toxin domain Block V of adenylate cyclase toxin","short_name":"RTX Block V","sequence":"MRGSHHHHHHGSHMELGASGSARDDVLIGDAGANVLNGLAGNDVLSGGAGDDVLLGDEGSDLLSGDAGNDDLFGGQGDDTYLFGVGYGHDTIYESGGGHDTIRINAGADQLWFARQGNDLEIRILGTDDALTVHDWYRDADHRVEIIHAANQAVDQAGIEKLVEAMAQYPDEFTSLEKLN*","organism":"Bordetella pertussis","uniprot_code":"P0DKX7","uniprot_range_first":1529,"uniprot_range_last":1680,"oligomerization":"monomer","molecular_type":"protein","uniprot_sequence":"MQQSHQAGYANAADRESGIPAAVLDGIKAVAKEKNATLMFRLVNPHSTSLIAEGVATKGL\nGVHAKSSDWGLQAGYIPVNPNLSKLFGRAPEVIARADNDVNSSLAHGHTAVDLTLSKERL\nDYLRQAGLVTGMADGVVASNHAGYEQFEFRVKETSDGRYAVQYRRKGGDDFEAVKVIGNA\nAGIPLTADIDMFAIMPHLSNFRDSARSSVTSGDSVTDYLARTRRAASEATGGLDRERIDL\nLWKIARAGARSAVGTEARRQFRYDGDMNIGVITDFELEVRNALNRRAHAVGAQDVVQHGT\nEQNNPFPEADEKIFVVSATGESQMLTRGQLKEYIGQQRGEGYVFYENRAYGVAGKSLFDD\nGLGAAPGVPSGRSKFSPDVLETVPASPGLRRPSLGAVERQDSGYDSLDGVGSRSFSLGEV\nSDMAAVEAAELEMTRQVLHAGARQDDAEPGVSGASAHWGQRALQGAQAVAAAQRLVHAIA\nLMTQFGRAGSTNTPQEAASLSAAVFGLGEASSAVAETVSGFFRGSSRWAGGFGVAGGAMA\nLGGGIAAAVGAGMSLTDDAPAGQKAAAGAEIALQLTGGTVELASSIALALAAARGVTSGL\nQVAGASAGAAAGALAAALSPMEIYGLVQQSHYADQLDKLAQESSAYGYEGDALLAQLYRD\nKTAAEGAVAGVSAVLSTVGAAVSIAAAASVVGAPVAVVTSLLTGALNGILRGVQQPIIEK\nLANDYARKIDELGGPQAYFEKNLQARHEQLANSDGLRKMLADLQAGWNASSVIGVQTTEI\nSKSALELAAITGNADNLKSVDVFVDRFVQGERVAGQPVVLDVAAGGIDIASRKGERPALT\nFITPLAAPGEEQRRRTKTGKSEFTTFVEIVGKQDRWRIRDGAADTTIDLAKVVSQLVDAN\nGVLKHSIKLDVIGGDGDDVVLANASRIHYDGGAGTNTVSYAALGRQDSITVSADGERFNV\nRKQLNNANVYREGVATQTTAYGKRTENVQYRHVELARVGQLVEVDTLEHVQHIIGGAGND\nSITGNAHDNFLAGGSGDDRLDGGAGNDTLVGGEGQNTVIGGAGDDVFLQDLGVWSNQLDG\nGAGVDTVKYNVHQPSEERLERMGDTGIHADLQKGTVEKWPALNLFSVDHVKNIENLHGSR\nLNDRIAGDDQDNELWGHDGNDTIRGRGGDDILRGGLGLDTLYGEDGNDIFLQDDETVSDD\nIDGGAGLDTVDYSAMIHPGRIVAPHEYGFGIEADLSREWVRKASALGVDYYDNVRNVENV\nIGTSMKDVLIGDAQANTLMGQGGDDTVRGGDGDDLLFGGDGNDMLYGDAGNDTLYGGLGD\nDTLEGGAGNDWFGQTQAREHDVLRGGDGVDTVDYSQTGAHAGIAAGRIGLGILADLGAGR\nVDKLGEAGSSAYDTVSGIENVVGTELADRITGDAQANVLRGAGGADVLAGGEGDDVLLGG\nDGDDQLSGDAGRDRLYGEAGDDWFFQDAANAGNLLDGGDGRDTVDFSGPGRGLDAGAKGV\nFLSLGKGFASLMDEPETSNVLRNIENAVGSARDDVLIGDAGANVLNGLAGNDVLSGGAGD\nDVLLGDEGSDLLSGDAGNDDLFGGQGDDTYLFGVGYGHDTIYESGGGHDTIRINAGADQL\nWFARQGNDLEIRILGTDDALTVHDWYRDADHRVEIIHAANQAVDQAGIEKLVEAMAQYPD\nPGAAAAAPPAARVPDTLMQSLAVNWR","mw":19.1,"total_mw":19.1,"number_molecules":1,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":""}],"buffer":{"name":"50 mM Tris, 5 mM DTT, 10 mM BaCl2","concentration_unit":null,"comment":null,"additive":null,"concentration":null,"pka":null,"ph":7.5,"deuteration":null},"purity_method":null,"name":"Repeats-in-toxin domain Block V of Bifunctional hemolysin/adenylate cyclase, 10 mM BaCl2","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[{"affiliation":[{"short_name":null,"address":"Stanford, California, USA","full_name":"Stanford University","webpage":"https://www.stanford.edu/"}],"contributor_name":"Alana","contributor_surname":"Gudinas","orcid":"https://orcid.org/0000-0003-2859-7868"}],"concentration_method":null,"concentration_unit":null,"date":"2024-08-01","storage_temperature":23.0,"cell_temperature":21.9,"exposure_time":2.0,"number_of_frames":1,"wavelength":0.1127,"sample_detector_distance":1.7,"concentration_min":null,"concentration_max":10.0,"sample_volume":null,"flow_rate":null,"s_min":0.066,"s_max":5.025,"total_exposure_time":null,"seccolumn":null},"fits":[{"models":[{"model_plot":null,"software":"DENSS","pdb_link":[],"model_title":null,"type_of_model":"density","software_version":null,"model_data":"https://www.sasbdb.org/media/pdb_file/SASDXX8_fit1_model1.mrc","model_mw":null,"bead_radius":null,"log":null,"symmetry":null,"comment":null,"user":1267}],"fit_unit":"1/A","fit_plot":"https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDXX8_fit1_fixed_fit_img.png","software":"DENSS","chi_square_value":5.1713,"p_value":0.0,"fit_residual_plot":"SASDXX8_fit1_fitresiduals_img.png","fit_data":"https://www.sasbdb.org/media/fitting_files/SASDXX8_fit1.fit","fit_log":null,"software_version":null,"description":""}],"estimated_volume_method":null,"pddf_software":"ATSAS GNOM","pddf_software_version":null,"i0_calibration_standard":null,"description":null,"experiment_description":"Synchrotron SAXS data from solutions of Disordered repeats-in-toxin domain Block V of Bifunctional hemolysin/adenylate cyclase in 50 mM Tris, 5 mM DTT, pH 7.5 were collected on the BL4-2 beam line at the Stanford Synchrotron Radiation Lightsource (SSRL) storage ring (Menlo Park, CA, USA) using a Pilatus3 X 1M detector at a sample-detector distance of 1.7 m and at a wavelength of λ = 0.1127 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 10.00 mg/ml was measured at 21.9°C. 500 frames were collected with an exposure time of 2 seconds. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent blank was subtracted.","tags":[],"intensity_unit":"arbitrary","experimental_mw":18.7,"experimental_mw_error":null,"guinier_i0_mw":null,"guinier_i0_mw_error":null,"porod_mw":22.0,"porod_mw_error":null,"pddf_i0":147.0,"pddf_i0_error":null,"guinier_i0":146.021,"guinier_i0_error":null,"pddf_rg":2.79,"pddf_rg_error":null,"guinier_rg":2.659,"guinier_rg_error":0.083,"pddf_dmax":11.7,"pddf_dmax_error":null,"porod_volume":35.0,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":2,"guinier_point_last":61,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDXX8_datloglog_img.png","symmetry":null,"last_modified":"2025-10-16T14:21:42.661720+02:00","bragg_peak":[]}