{"code":"SASDXY4","status":"Published","type_of_curve":"SEC-SAS","angular_unit":"1/A","project":{"title":"Structural, biophysical and biochemical studies of CAZYmes involved in exopolysaccharides degradation of microbial biofilms","publication":{"title":"Structural, biophysical and biochemical studies of CAZYmes involved in exopolysaccharides degradation of microbial biofilms","author_list":"Amanda Freitas Cruz","journal":"Universidade de São Paulo","doi":null,"pmid":null,"published_date":"2025 Sep 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marcescens PgaB withoud two disordered regions in the N- and C-terminals."}],"buffer":{"name":"phosphate-buffered saline","concentration_unit":null,"comment":null,"additive":null,"concentration":null,"pka":null,"ph":7.4,"deuteration":null},"purity_method":null,"name":"Serratia marcescens truncated Poly-beta-1,6-N-acetyl-D-glucosamine N-deacetylase PgaB","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[{"affiliation":[{"short_name":null,"address":"São Paulo, SP, Brazil","full_name":"Instituto de Física, Universidade de São Paulo","webpage":""}],"contributor_name":"Amanda","contributor_surname":"Freitas Cruz","orcid":""}],"concentration_method":null,"concentration_unit":null,"date":"2025-02-03","storage_temperature":-80.0,"cell_temperature":20.0,"exposure_time":0.1,"number_of_frames":4,"wavelength":0.1127,"sample_detector_distance":1.7,"concentration_min":null,"concentration_max":8.0,"sample_volume":0.05,"flow_rate":0.05,"s_min":0.06,"s_max":5.045,"total_exposure_time":null,"seccolumn":1},"fits":[{"models":[{"model_plot":"https://www.sasbdb.org/media/pdb_file/images/SASDXY4_fit1_model1_img.png","software":"AlphaFold","pdb_link":[],"model_title":null,"type_of_model":"atomic","software_version":null,"model_data":"https://www.sasbdb.org/media/pdb_file/SASDXY4_fit1_model1.pdb","model_mw":69.1,"bead_radius":null,"log":null,"symmetry":"P1","comment":null,"user":1234}],"fit_unit":"1/A","fit_plot":"https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDXY4_fit1_fixed_fit_img.png","software":"CRYSOL","chi_square_value":4.273,"p_value":0.0,"fit_residual_plot":"SASDXY4_fit1_fitresiduals_img.png","fit_data":"https://www.sasbdb.org/media/fitting_files/SASDXY4_fit1.fit","fit_log":null,"software_version":null,"description":""}],"estimated_volume_method":null,"pddf_software":"ATSAS GNOM","pddf_software_version":"4.1","i0_calibration_standard":null,"description":null,"experiment_description":"Synchrotron SAXS data from solutions of truncated PgaB in phosphate-buffered saline, pH 7.4 were collected on the BL4-2 beam line at the Stanford Synchrotron Radiation Lightsource (SSRL; Menlo Park, CA, USA) using a Pilatus3 X 1M detector at a sample-detector distance of 1.7 m and at a wavelength of λ = 0.1127 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 0.05 μl sample at 8 mg/ml was injected at a 0.05 ml/min flow rate onto a GE Superdex 200 Increase 10/300 column at 20°C. Four successive 0.100 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.","tags":[],"intensity_unit":"arbitrary","experimental_mw":65.7,"experimental_mw_error":null,"guinier_i0_mw":null,"guinier_i0_mw_error":null,"porod_mw":65.7,"porod_mw_error":null,"pddf_i0":17.64,"pddf_i0_error":null,"guinier_i0":17.5918,"guinier_i0_error":null,"pddf_rg":2.77,"pddf_rg_error":null,"guinier_rg":2.752,"guinier_rg_error":0.028,"pddf_dmax":9.01,"pddf_dmax_error":null,"porod_volume":101.54,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":12,"guinier_point_last":78,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDXY4_datloglog_img.png","symmetry":null,"last_modified":"2025-11-24T10:26:42.360507+01:00","bragg_peak":[]}