{"code":"SASDYR9","status":"Published","type_of_curve":"SEC-SAS","angular_unit":"1/nm","project":{"title":"ATPLyzer—An Advanced Ratiometric Multi‐Color Biosensor for Long‐Term Monitoring of ATP Dynamics","publication":{"title":"ATPLyzer—An Advanced Ratiometric Multi‐Color Biosensor for Long‐Term Monitoring of ATP Dynamics","author_list":"Papadopoulos A, Kaiser C, Schlumpberger P, Eßer J, Reiners J, Gertzen C, Grossmann G, Smits S","journal":"Advanced Sensor Research","doi":"10.1002/adsr.70171","pmid":null,"published_date":"2026 Jun 27"},"status":"released","submitted_date":"2026-03-09","released_date":"2026-06-09"},"pddf_data":"https://www.sasbdb.org/media/p_of_R_files/SASDYR9.out","intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDYR9.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDYR9_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDYR9_kratky_img.png","pddf_plot":"https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDYR9_pofr_img.png","intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDYR9_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDYR9.sascif","experiment":{"instrument":{"detector":{"type":null,"name":"Pilatus3 2M","resolution":0.172},"name":"ESRF","city":"Grenoble","country":"France","beamline_name":"BM29","beam_geometry":"point","type_of_source":"X-ray synchrotron","point_source":null,"line_collimation":null,"sample_path_length":null,"line_collimation_slitlength":null,"line_collimation_integrationwidth":null,"xray_energy":null,"beam_profile_ah":null,"beam_profile_al":null},"sample":{"molecule":[{"long_name":"Ratiometric matryoshka multi-colour biosensor for ATP","short_name":"ATPLyzer GO 1.7m ATP","sequence":"MRGSHHHHHHGMASMTGGQQMGRDLYDDDDKDPMKTVKVNITTPDGPVYDADIEMVSVRAESGDLGILPGHIPTKAPLKIGAVRLKKDGQTEMVAVSGGTVEVRPDHVTINAQAAETAEGIDKERAEAARQRAQERLNSQTRYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLEFVTAAGITHGMDELYGGTMVSKGEENNMAIIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGFQTVKLKVTKGGPLPFAWDILSPQFTYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGMEASSERMYPEDGALKGEDKLRLKLKDGGHYTSEVKTTYKAKKPVQLPGAYIVDIKLDITSHNEDYTIVEQYERAEGRHSTGGMDELYKGGSASQGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNLGDIDFKRAELALKRAMNRLSVAEMK","organism":"synthetic construct","uniprot_code":null,"uniprot_range_first":null,"uniprot_range_last":null,"oligomerization":"monomer","molecular_type":"protein","uniprot_sequence":"","mw":72.205,"total_mw":72.205,"number_molecules":1,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":"The sensor contains different domains: • ε subunit of the bacterial FoF1 ATPase from Bacillus spec. • superfolder circularly permutated GFP (cpsfGFP (reporter fluorophore) from Aequorea victoria) • LSSmApple (reference fluorophore) from Discosoma sp. and is constructed as: 1–39 N-Terminal 10xHis, T7tag, enterokinase site/XpressTag, 40–147 εFoF1-N-terminus of B. subtilis, 148–151 PASH Linker, 152–240 cpsfGFP-C-terminus, 241–243 GGT Linker, 244–478 LSSmApple, 479–481 GGS Linker, 482–625 cpsfGFP-N-terminus, 626–629 FNNP Linker, 630–708 εFoF1-N-terminus of B. PS3-C-terminus."}],"buffer":{"name":"50 mM MOPS, 100 mM KCl, 1 mM MgCl2, 5mM ATP,","concentration_unit":null,"comment":null,"additive":null,"concentration":null,"pka":null,"ph":7.0,"deuteration":null},"purity_method":null,"name":"ATPLyzer GO 1.7 m ATP","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[{"affiliation":[{"short_name":null,"address":"Düsseldorf, Germany","full_name":"Heinrich Heine University Düsseldorf","webpage":"https://www.uni-duesseldorf.de"}],"contributor_name":"Jens","contributor_surname":"Reiners","orcid":""}],"concentration_method":null,"concentration_unit":null,"date":"2023-11-30","storage_temperature":10.0,"cell_temperature":10.0,"exposure_time":3.0,"number_of_frames":800,"wavelength":0.099,"sample_detector_distance":2.812,"concentration_min":null,"concentration_max":4.9,"sample_volume":100.0,"flow_rate":0.6,"s_min":0.085,"s_max":4.012,"total_exposure_time":null,"seccolumn":1},"fits":[{"models":[{"model_plot":"https://www.sasbdb.org/media/pdb_file/images/SASDYR9_fit1_model1_img.png","software":"EOM/RANCH","pdb_link":[],"model_title":null,"type_of_model":"mix","software_version":null,"model_data":"https://www.sasbdb.org/media/pdb_file/SASDYR9_fit1_model1.pdb","model_mw":72.37,"bead_radius":1.9,"log":"https://www.sasbdb.org/media/log_files/test00.log","symmetry":"P1","comment":null,"user":402}],"fit_unit":"1/A","fit_plot":"https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDYR9_fit1_fixed_fit_img.png","software":"CRYSOL","chi_square_value":0.995,"p_value":0.7803,"fit_residual_plot":"SASDYR9_fit1_fitresiduals_img.png","fit_data":"https://www.sasbdb.org/media/fitting_files/SASDYR9_fit1.fit","fit_log":"https://www.sasbdb.org/media/fitting_files/log_files/test00.log","software_version":"C R Y S O L    Version 2.8.3","description":""}],"estimated_volume_method":null,"pddf_software":"ATSAS GNOM","pddf_software_version":"5.0","i0_calibration_standard":null,"description":"Please read the molecule description by clicking the molecule link.  Collected raw SAXS data from the ESRF (proposal ID MX-2485) can be found under: https://doi.org/10.15151/ESRF-DC-2387389777","experiment_description":null,"tags":[],"intensity_unit":"1/cm","experimental_mw":72.22,"experimental_mw_error":null,"guinier_i0_mw":null,"guinier_i0_mw_error":null,"porod_mw":66.0,"porod_mw_error":null,"pddf_i0":30.39,"pddf_i0_error":null,"guinier_i0":30.3711,"guinier_i0_error":null,"pddf_rg":3.747,"pddf_rg_error":null,"guinier_rg":3.67,"guinier_rg_error":0.02,"pddf_dmax":12.02,"pddf_dmax_error":null,"porod_volume":106.16,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":1,"guinier_point_last":53,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDYR9_datloglog_img.png","symmetry":null,"last_modified":"2026-06-30T10:44:46.834115+02:00","bragg_peak":[]}