{"code":"SASDYU3","status":"Published","type_of_curve":"SEC-SAS","angular_unit":"1/A","project":{"title":"Solution characterization of TraW, a regulatory protein of the F plasmid type 4 secretion system","publication":{"title":"Solution characterization of TraW, a regulatory protein of the F plasmid type 4 secretion system","author_list":"Rodriguez C, Audette G","journal":"Structural Dynamics","doi":"10.1063/4.0001201","pmid":null,"published_date":"2026 Mar 17"},"status":"released","submitted_date":"2025-12-10","released_date":"2026-03-18"},"pddf_data":"https://www.sasbdb.org/media/p_of_R_files/SASDYU3.out","intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDYU3.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDYU3_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDYU3_kratky_img.png","pddf_plot":"https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDYU3_pofr_img.png","intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDYU3_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDYU3.sascif","experiment":{"instrument":{"detector":{"type":null,"name":"Eiger2 XE 9M","resolution":72.0},"name":"Advanced Photon Source (APS), Argonne National Laboratory","city":"Lemont, IL","country":"USA","beamline_name":"BioCAT 18ID","beam_geometry":null,"type_of_source":"X-ray synchrotron","point_source":null,"line_collimation":null,"sample_path_length":null,"line_collimation_slitlength":null,"line_collimation_integrationwidth":null,"xray_energy":null,"beam_profile_ah":null,"beam_profile_al":null},"sample":{"molecule":[{"long_name":"TraW (Δ1-67)","short_name":null,"sequence":"LRPPAVPGIGRTEKYGSRLFDPSVRLAADIRDNEGRVFARQGEVMNPLQYVPFNQTLYFINGDDPAQVAWMKRQTPPTLESKIILVQGSIPEMQKSLDSRVYFDQNGVLCQRLGIDQVPARVSAVPGDRFLKVEFIPAEEGRK","organism":"Escherichia coli (strain K12)","uniprot_code":"P18472","uniprot_range_first":68,"uniprot_range_last":210,"oligomerization":"monomer","molecular_type":"protein","uniprot_sequence":"MRCRGLIALLIWGQSVAAADLGTWGDLWPVKEPDMLTVIMQRLTALEQSGEMGRKMDAFK\nERVIRNSLRPPAVPGIGRTEKYGSRLFDPSVRLAADIRDNEGRVFARQGEVMNPLQYVPF\nNQTLYFINGDDPAQVAWMKRQTPPTLESKIILVQGSIPEMQKSLDSRVYFDQNGVLCQRL\nGIDQVPARVSAVPGDRFLKVEFIPAEEGRK","mw":19.9,"total_mw":19.9,"number_molecules":1,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":""}],"buffer":{"name":"20 mM HEPES, 100 mM NaCl, 5% glycerol","concentration_unit":null,"comment":null,"additive":null,"concentration":null,"pka":null,"ph":7.0,"deuteration":null},"purity_method":null,"name":"N-terminal truncation mutant, of the conjugative protein of the F plasmid Type IV Secretion System, TraW.","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[],"concentration_method":null,"concentration_unit":null,"date":"2022-11-17","storage_temperature":4.0,"cell_temperature":22.0,"exposure_time":0.7,"number_of_frames":3175,"wavelength":0.1033,"sample_detector_distance":3.7,"concentration_min":null,"concentration_max":13.0,"sample_volume":350.0,"flow_rate":0.5,"s_min":0.029,"s_max":4.171,"total_exposure_time":null,"seccolumn":3},"fits":[{"models":[{"model_plot":"https://www.sasbdb.org/media/pdb_file/images/SASDYU3_fit1_model1_img.png","software":"DAMMIN","pdb_link":[],"model_title":null,"type_of_model":"dummy","software_version":"3.2.1","model_data":"https://www.sasbdb.org/media/pdb_file/SASDYU3_fit1_model1.cif","model_mw":null,"bead_radius":null,"log":"https://www.sasbdb.org/media/log_files/refine_MUT80.log","symmetry":null,"comment":null,"user":1301}],"fit_unit":"1/A","fit_plot":"https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDYU3_fit1_fixed_fit_img.png","software":"DAMMIN","chi_square_value":1.271,"p_value":0.0859,"fit_residual_plot":"SASDYU3_fit1_fitresiduals_img.png","fit_data":"https://www.sasbdb.org/media/fitting_files/SASDYU3_fit1.fir","fit_log":"https://www.sasbdb.org/media/fitting_files/log_files/refine_MUT80.log","software_version":"3.2.1","description":""}],"estimated_volume_method":null,"pddf_software":"ATSAS GNOM","pddf_software_version":"3.2.1","i0_calibration_standard":null,"description":"Synchrotron SAXS data from solutions of a N-terminal truncation mutant of TraW in 20 mM HEPES, 100 mM NaCl, 5% glycerol, pH 7 were collected on the BioCAT 18ID beam line at the Advanced Photon Source (APS; Argonne National Laboratory USA) using a Eiger2 XE 9M detector at a sample-detector distance of 3.7 m and at a wavelength of λ = 0.1033 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 350.00 μl sample at 13 mg/ml was injected at a 0.5 ml/min flow rate onto a GE Superdex 75 Increase 10/300 column at 22°C. 3175 successive 0.500 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.","experiment_description":"-","tags":[],"intensity_unit":null,"experimental_mw":20.0,"experimental_mw_error":null,"guinier_i0_mw":null,"guinier_i0_mw_error":null,"porod_mw":20.7,"porod_mw_error":null,"pddf_i0":1.613,"pddf_i0_error":0.0017,"guinier_i0":1.6174,"guinier_i0_error":null,"pddf_rg":2.18,"pddf_rg_error":0.074,"guinier_rg":2.18,"guinier_rg_error":0.093,"pddf_dmax":8.0,"pddf_dmax_error":null,"porod_volume":null,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":13,"guinier_point_last":321,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDYU3_datloglog_img.png","symmetry":null,"last_modified":"2026-03-18T13:59:44.111420+01:00","bragg_peak":[]}