{"code":"SASDZ88","status":"Published","type_of_curve":"SEC-SAS","angular_unit":"1/nm","project":{"title":"A chimeric, half-life extended lysin with a unique mode of action","publication":{"title":"A chimeric, half-life extended lysin with a unique mode of action","author_list":"Visram Z, Kestemont D, Bauer B, Qiao R, Durica-Mitic S, Majoros-Hashempour A, Schmidt J, Berdaguer R, Krey K, Mutti M, Zerbs M, von Freyberg M, Corsini L, Badarau A","journal":"Frontiers in Microbiology","doi":"10.3389/fmicb.2026.1880134","pmid":null,"published_date":"2026 Aug 21"},"status":"released","submitted_date":"2026-05-13","released_date":"2026-07-23"},"pddf_data":"https://www.sasbdb.org/media/p_of_R_files/SASDZ88.dat","intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDZ88.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDZ88_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDZ88_kratky_img.png","pddf_plot":"https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDZ88_pofr_img.png","intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDZ88_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDZ88.sascif","experiment":{"instrument":{"detector":{"type":null,"name":"Pilatus 6M","resolution":null},"name":"PETRA III","city":"DESY; Hamburg","country":"Germany","beamline_name":"EMBL P12","beam_geometry":null,"type_of_source":"X-ray synchrotron","point_source":null,"line_collimation":null,"sample_path_length":null,"line_collimation_slitlength":null,"line_collimation_integrationwidth":null,"xray_energy":null,"beam_profile_ah":null,"beam_profile_al":null},"sample":{"molecule":[{"long_name":"Fc-L2a-CHAP(alt)-LSN(HEK)","short_name":"L2184","sequence":"GPEPKSSDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSRDELTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGKAEAAAKEAAAKEAAAKEAAAKEAAAKEAAAKEAAAKALEAEAAAKEAAAKEAAAKEAAAKEAAAKEAAAKEAAAKAKPETVSGNEKSNSGSSTLNYLKSLEGKGWDFDGSFGWQCFDLVNVYWNHLYGHGLKGYYAKDIPYANNFNSEAKIYHNTPTFKAEPGDLVVFSGRYGGGYGHTAIVLNGNYDGNLQKFQSLDQNWNGGGERKQEVAHKVVHNYENDMIFIRPFKKAGGAATHEHSAQWLNNYKKGYGYGPYPLGINGGMHYGVDFFMNIGTPVKAISSGKIVEAGWSNYGGGNQIGLIENDGVHRQWYMHLSKYNVKVGDYVKAGQIIGWSGSTGYSTAPHLHFQRMVNSFSNPTAQDPMPFLKSAGYGKAGGTVTPTPNTGWKTNKYGTLYKSESASFTPNTDIITRTTGPFRSMPQSGVLKAGQTIHYDEVMKQDGHVWVGYTGNSGQRIYLPVRTWQKSTNTLGVLWGTIK","organism":"synthetic construct","uniprot_code":null,"uniprot_range_first":null,"uniprot_range_last":null,"oligomerization":"dimer","molecular_type":"protein","uniprot_sequence":"","mw":77.974,"total_mw":155.948,"number_molecules":2,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":"Human IgG1 Fc fused via a helical linker to a genetic fusion of the CHAP domain of an engineered derivative  of S.aureus LytN and an engineered derivative of S.simulans lysostaphin."}],"buffer":{"name":"20 mM HEPES, 150 mM NaCl, pH 7.0","concentration_unit":null,"comment":null,"additive":null,"concentration":null,"pka":null,"ph":7.0,"deuteration":null},"purity_method":null,"name":"Synthetic fusion protein comprising human IgG1 Fc, the CHAP domain of engineered Staphylococcus aureus LytN, and engineered Staphylococcus simulans lysostaphin (Fc-L2a-CHAP(alt)-LSN(HEK))","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[{"affiliation":[{"short_name":null,"address":null,"full_name":"BioNTech R&D Austria GmbH","webpage":null}],"contributor_name":"Adriana","contributor_surname":"Badarau","orcid":""}],"concentration_method":null,"concentration_unit":null,"date":"2025-04-06","storage_temperature":25.0,"cell_temperature":25.0,"exposure_time":0.5,"number_of_frames":1800,"wavelength":0.124,"sample_detector_distance":3.0,"concentration_min":null,"concentration_max":5.0,"sample_volume":25.0,"flow_rate":0.35,"s_min":0.022,"s_max":1.283,"total_exposure_time":null,"seccolumn":49},"fits":[],"estimated_volume_method":null,"pddf_software":"Other","pddf_software_version":"GNOM","i0_calibration_standard":null,"description":null,"experiment_description":null,"tags":[],"intensity_unit":"arbitrary","experimental_mw":155.0,"experimental_mw_error":null,"guinier_i0_mw":null,"guinier_i0_mw_error":null,"porod_mw":null,"porod_mw_error":null,"pddf_i0":0.09492966,"pddf_i0_error":null,"guinier_i0":0.0914397,"guinier_i0_error":null,"pddf_rg":9.963,"pddf_rg_error":null,"guinier_rg":8.9,"guinier_rg_error":0.2,"pddf_dmax":40.0,"pddf_dmax_error":null,"porod_volume":null,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":12,"guinier_point_last":45,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDZ88_datloglog_img.png","symmetry":null,"last_modified":"2026-08-24T09:04:10.134341+02:00","bragg_peak":[]}