{"code":"SASDZD8","status":"Published","type_of_curve":"SEC-SAS","angular_unit":"1/A","project":{"title":"Schistosoma mansoni Venom Allergen-like protein 11 (SmVAL11) in complex with cognate polyclonal antibody (pAb anti-SmVAL11)","publication":null,"status":"released","submitted_date":"2026-05-18","released_date":"2026-07-28"},"pddf_data":"https://www.sasbdb.org/media/p_of_R_files/SASDZD8.out","intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDZD8.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDZD8_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDZD8_kratky_img.png","pddf_plot":"https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDZD8_pofr_img.png","intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDZD8_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDZD8.sascif","experiment":{"instrument":{"detector":{"type":null,"name":"Eiger 4M","resolution":75.0},"name":"SOLEIL","city":"Saint-Aubin","country":"France","beamline_name":"SWING","beam_geometry":null,"type_of_source":"X-ray synchrotron","point_source":null,"line_collimation":null,"sample_path_length":null,"line_collimation_slitlength":null,"line_collimation_integrationwidth":null,"xray_energy":null,"beam_profile_ah":null,"beam_profile_al":null},"sample":{"molecule":[{"long_name":"Venom Allergen-like protein 11","short_name":"SmVAL11","sequence":"MSRLDDFREDCFRAHNEKRLLHGVCALRHSLALDKTAQDWAEALLSEDGIKNSPLSSRGEVGESISVRTSTGTHVDMQGHEVVNTWHSDAENYNYENGKGPAGNFTQLVWSSTREVGFGKACGPGKCVVVAHYRPPGNVLGRYLENVFRPKKSVKDVKQPIRNTFALNNDTPKTVITETLTESDGKQYSVRREISDLTDDKGKNKKLYLVGQGKKVEYFSCCFNNRTRRCINEVYTDACKEQKKAKSSISPDGHLVDNSKKLQESIHSVVQLHNQYRSQHGSNPLVLDQNLSNMAQQWADHLLQQSHLSNSGYVYRGMKVGENLGSRWSNGPMELNCKDLIEHWYQESGKYKFNSEPDSIQGIGNFTQIVWTSSERIGVGIAIQSYKSGEDLHKDSKMILVCLYHPPGNVISQFQNNVKKAIK","organism":"Schistosoma mansoni","uniprot_code":"Q1WL54","uniprot_range_first":1,"uniprot_range_last":423,"oligomerization":"other","molecular_type":"protein","uniprot_sequence":"MSRLDDFREDCFRAHNEKRLLHGVCALRHSLALDKTAQDWAEALLSEDGIKNSPLSSRGE\nVGESISVRTSTGTHVDMQGHEVVNTWHSDAENYNYENGKGPAGNFTQLVWSSTREVGFGK\nACGPGKCVVVAHYRPPGNVLGRYLENVFRPKKSVKDVKQPIRNTFALNNDTPKTVITETL\nTESDGKQYSVRREISDLTDDKGKNKKLYLVGQGKKVEYFSCCFNNRTRRCINEVYTDACK\nEQKKAKSSISPDGHLVDNSKKLQESIHSVVQLHNQYRSQHGSNPLVLDQNLSNMAQQWAD\nHLLQQSHLSNSGYVYRGMKVGENLGSRWSNGPMELNCKDLIEHWYQESGKYKFNSEPDSI\nQGIGNFTQIVWTSSERIGVGIAIQSYKSGEDLHKDSKMILVCLYHPPGNVISQFQNNVKK\nAIK","mw":47.0,"total_mw":47.0,"number_molecules":3,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":"Schistosoma mansoni Venom Allergen-like protein 11 (SmVAL11) is a 47 kDa protein composed by two symmetrical domains  separated by a flexible linker. The latter, in our experience, undergoes autocleavage in vitro, with an unknown mechanism."},{"long_name":"Polyclonal antibody anti-SmVAL11","short_name":"pAb anti-SmVAL11","sequence":"A","organism":"Lagomorph","uniprot_code":null,"uniprot_range_first":null,"uniprot_range_last":null,"oligomerization":"monomer","molecular_type":"protein","uniprot_sequence":"","mw":0.089,"total_mw":140.0,"number_molecules":1,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":"Polyclonal antibody raised against SmVAL11 in rabbit."}],"buffer":{"name":"25 mM MES, 125 mM NaCl, 1 mM EDTA","concentration_unit":null,"comment":null,"additive":null,"concentration":null,"pka":null,"ph":6.8,"deuteration":null},"purity_method":null,"name":"Schistosoma mansoni Venom Allergen-like protein isoform 11 (SmVAL11) in complex with pAb anti-SmVAL11, ratio 1:1 (2nd peak)","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[{"affiliation":[{"short_name":null,"address":null,"full_name":"University of Lyon","webpage":null}],"contributor_name":"Adriana Erica","contributor_surname":"Miele","orcid":"http://orcid.org/0000-0002-4637-2606"}],"concentration_method":null,"concentration_unit":null,"date":"2025-01-22","storage_temperature":4.0,"cell_temperature":20.0,"exposure_time":1.0,"number_of_frames":2600,"wavelength":0.76,"sample_detector_distance":2.0,"concentration_min":null,"concentration_max":2.7,"sample_volume":45.0,"flow_rate":0.3,"s_min":0.068,"s_max":5.529,"total_exposure_time":null,"seccolumn":null},"fits":[],"estimated_volume_method":null,"pddf_software":"ATSAS GNOM","pddf_software_version":"5.0","i0_calibration_standard":null,"description":"SmVAL11 in complex with its cognate pAb at a 1:1 ratio was analyzed by SEC-SAXS. Spectral deconvolution was performed using Evolving Factor Analysis (EFA), revealing two distinct peaks. The present analysis focuses on the second peak.","experiment_description":null,"tags":[],"intensity_unit":null,"experimental_mw":146.8,"experimental_mw_error":null,"guinier_i0_mw":null,"guinier_i0_mw_error":null,"porod_mw":169.2,"porod_mw_error":null,"pddf_i0":1.41,"pddf_i0_error":null,"guinier_i0":1.40187,"guinier_i0_error":null,"pddf_rg":4.99,"pddf_rg_error":null,"guinier_rg":4.874,"guinier_rg_error":0.019,"pddf_dmax":16.4,"pddf_dmax_error":null,"porod_volume":204.0,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":10,"guinier_point_last":39,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDZD8_datloglog_img.png","symmetry":null,"last_modified":"2026-07-28T14:42:19.875272+02:00","bragg_peak":[]}