{"code":"SASDZM8","status":"Published","type_of_curve":"SEC-SAS","angular_unit":"1/A","project":{"title":"Schistosoma mansoni Venom Allergen-like protein 13 (SmVAL13) in complex with cognate polyclonal antibody (pAb anti-SmVAL13)","publication":null,"status":"released","submitted_date":"2026-05-18","released_date":"2026-07-28"},"pddf_data":"https://www.sasbdb.org/media/p_of_R_files/SASDZM8.out","intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDZM8.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDZM8_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDZM8_kratky_img.png","pddf_plot":"https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDZM8_pofr_img.png","intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDZM8_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDZM8.sascif","experiment":{"instrument":{"detector":{"type":null,"name":"Eiger 4M","resolution":75.0},"name":"SOLEIL","city":"Saint-Aubin","country":"France","beamline_name":"SWING","beam_geometry":null,"type_of_source":"X-ray synchrotron","point_source":null,"line_collimation":null,"sample_path_length":null,"line_collimation_slitlength":null,"line_collimation_integrationwidth":null,"xray_energy":null,"beam_profile_ah":null,"beam_profile_al":null},"sample":{"molecule":[{"long_name":"Venom allergen-like protein 13","short_name":"SmVAL13","sequence":"MVDEQLNHDA LNEHNRLRAL HGCPPLKYDR RLAREAQAWA ENLARLKIMK HSICDEYGEN\r\nLASAQSTGKA EMTGARATRN WYDEIHYHNF NKQFQSQSGH FTQLIWKNTS KAGFGIQHSV\r\nDGHHVFIVGR YEPPGNVNGQ FLENVPPPIH GQSTPKSKVP SYKHNEQNGP RRTYQDELVI\r\nVRETDRKDHN GSNHITLIDS SKRSRSDETI PNKNEVRIIR AEKKRQRRCA KRCSIM","organism":"Schistosoma mansoni","uniprot_code":"Q1WIQ5","uniprot_range_first":1,"uniprot_range_last":236,"oligomerization":"monomer","molecular_type":"protein","uniprot_sequence":"MVDEQLNHDALNEHNRLRALHGCPPLKYDRRLAREAQAWAENLARLKIMEHSICDEYGEN\nLASAQSTGKAEMTGARATRNWYDEIHYHNFNKQFQSQSGHFTQLIWKNTSKAGFGIQHSV\nDGHHVFIVGRYEPPGNVNGQFLENVPPPIHGQSTPKSKVPSYKHNEQNGPRRTYQDELVI\nVRETDRKDHNGSNHITLIDSSKRSRSDETIPNKNEVRIIRAEKKRQRRCAKRCSIM","mw":27.225,"total_mw":27.225,"number_molecules":1,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":"SCP/TAPS domain flanked by intrinsically disordered terminal regions"},{"long_name":"Polyclonal antibody anti-SmVAL13","short_name":"pAb anti-SmVAL13","sequence":"A","organism":"Lagomorph","uniprot_code":null,"uniprot_range_first":null,"uniprot_range_last":null,"oligomerization":"monomer","molecular_type":"protein","uniprot_sequence":"","mw":140.0,"total_mw":140.0,"number_molecules":1,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":"Polyclonal antibody anti-SmVAL13 raised in rabbit"}],"buffer":{"name":"25 mM MES, 125 mM NaCl, 1 mM EDTA","concentration_unit":null,"comment":null,"additive":null,"concentration":null,"pka":null,"ph":6.8,"deuteration":null},"purity_method":null,"name":"Schistosoma mansoni Venom Allergen-like protein isoform 13 (SmVAL13) in complex with pAb anti-SmVAL13 in ratio 2:1 (1st peak)","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[{"affiliation":[{"short_name":null,"address":null,"full_name":"University of Lyon","webpage":null}],"contributor_name":"Adriana Erica","contributor_surname":"Miele","orcid":"http://orcid.org/0000-0002-4637-2606"}],"concentration_method":null,"concentration_unit":null,"date":"2025-01-22","storage_temperature":4.0,"cell_temperature":20.0,"exposure_time":1.0,"number_of_frames":2600,"wavelength":0.76,"sample_detector_distance":2.0,"concentration_min":null,"concentration_max":3.3,"sample_volume":45.0,"flow_rate":0.3,"s_min":0.05,"s_max":5.529,"total_exposure_time":null,"seccolumn":48},"fits":[],"estimated_volume_method":null,"pddf_software":"ATSAS GNOM","pddf_software_version":"5.0","i0_calibration_standard":null,"description":"SmVAL13 in complex with its cognate pAb at a 2:1 ratio was analyzed by SEC-SAXS. Spectral deconvolution was performed using Evolving Factor Analysis (EFA), revealing two distinct peaks. The present analysis focuses on the first peak.","experiment_description":null,"tags":[],"intensity_unit":null,"experimental_mw":646.8,"experimental_mw_error":null,"guinier_i0_mw":null,"guinier_i0_mw_error":null,"porod_mw":792.5,"porod_mw_error":null,"pddf_i0":0.5247,"pddf_i0_error":null,"guinier_i0":0.481362,"guinier_i0_error":null,"pddf_rg":10.51,"pddf_rg_error":null,"guinier_rg":9.041,"guinier_rg_error":0.311,"pddf_dmax":36.9,"pddf_dmax_error":null,"porod_volume":955.0,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":5,"guinier_point_last":19,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDZM8_datloglog_img.png","symmetry":null,"last_modified":"2026-07-28T14:41:05.276570+02:00","bragg_peak":[]}