{"code":"SASDZV5","status":"Published","type_of_curve":"SEC-SAS","angular_unit":"1/A","project":{"title":"The Rho guanine-nucleotide exchange factor P-Rex2 exhibits structural and regulatory features distinct from the related RhoGEF P-Rex1","publication":{"title":"The Rho guanine-nucleotide exchange factor P-Rex2 exhibits structural and regulatory features distinct from the related RhoGEF P-Rex1","author_list":"Anderson L, Marde R, Muma G, Nayak V, Phan C, Li S, Cash J","journal":"Journal of Biological Chemistry","doi":"10.1016/j.jbc.2026.113229","pmid":null,"published_date":"2026 Jul"},"status":"released","submitted_date":"2026-04-03","released_date":"2026-07-23"},"pddf_data":"https://www.sasbdb.org/media/p_of_R_files/SASDZV5.out","intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDZV5.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDZV5_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDZV5_kratky_img.png","pddf_plot":"https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDZV5_pofr_img.png","intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDZV5_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDZV5.sascif","experiment":{"instrument":{"detector":{"type":null,"name":"Pilatus3 X 2M","resolution":172.0},"name":"Advanced Light Source (ALS)","city":"Berkeley, CA","country":"USA","beamline_name":"12.3.1 (SIBYLS)","beam_geometry":"","type_of_source":"X-ray synchrotron","point_source":null,"line_collimation":null,"sample_path_length":null,"line_collimation_slitlength":null,"line_collimation_integrationwidth":null,"xray_energy":null,"beam_profile_ah":null,"beam_profile_al":null},"sample":{"molecule":[{"long_name":"Phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 2 DH/PH tandem","short_name":"P-Rex2 DH/PH tandem","sequence":"GAMDPMSEDSRGDSRAESAKDLEKQLRLRVCVLSELQKTERDYVGTLEFLVSAFLHRMNQCAASKVDKNVTEETVKMLFSNIEDILAVHKEFLKVVEECLHPEPNAQQEVGTCFLHFKDKFRIYDEYCSNHEKAQKLLLELNKIRTIRTFLLNCMLLGGRKNTDVPLEGYLVTPIQRICKYPLILKELLKRTPRKHSDYAAVMEALQAMKAVCSNINEAKRQMEKLEVLEEWQSHIEGWEGSNITDTCTEMLMCGVLLKISSGNIQERVFFLFDNLLVYCKRKHRRLKNSKASTDGHRYLFRGRINTEVMEVENVDDGTADFHSSGHIVVNGWKIHNTAKNKWFVCMAKTPEEKHEWFEAILKERERRKGLKLGMEQDTWVM","organism":"Homo 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P-Rex2 DH/PH tandem with residual linker (GAMDP) from N-terminal TEV cleavage"}],"buffer":{"name":"20 mM HEPES, 200 mM NaCl, 2 mM DTT, 1% glycerol","concentration_unit":null,"comment":null,"additive":null,"concentration":null,"pka":null,"ph":7.0,"deuteration":null},"purity_method":null,"name":"Human Phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger protein 2 DH/PH domain tandem (P-Rex2 DH/PH)","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[{"affiliation":[{"short_name":null,"address":null,"full_name":"University of California, Davis","webpage":null}],"contributor_name":"Lauren","contributor_surname":"Anderson","orcid":"http://lauanderson@ucdavis.edu"}],"concentration_method":null,"concentration_unit":null,"date":"2025-06-10","storage_temperature":null,"cell_temperature":null,"exposure_time":2.0,"number_of_frames":750,"wavelength":0.1127,"sample_detector_distance":2.1,"concentration_min":null,"concentration_max":2.0,"sample_volume":null,"flow_rate":0.65,"s_min":0.124,"s_max":4.707,"total_exposure_time":null,"seccolumn":17},"fits":[{"models":[{"model_plot":"https://www.sasbdb.org/media/pdb_file/images/SASDZV5_fit1_model1_img.png","software":"BILBOMD","pdb_link":[],"model_title":null,"type_of_model":"atomic","software_version":null,"model_data":"https://www.sasbdb.org/media/pdb_file/SASDZV5_fit1_model1.pdb","model_mw":43.75,"bead_radius":null,"log":null,"symmetry":null,"comment":null,"user":1310}],"fit_unit":"1/A","fit_plot":"https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDZV5_fit1_fitfoxs_img.png","software":"MultiFoXS","chi_square_value":0.679376,"p_value":0.0017,"fit_residual_plot":"SASDZV5_fit1_fitfoxsresiduals_img.png","fit_data":"https://www.sasbdb.org/media/fitting_files/SASDZV5_fit1.dat","fit_log":"https://www.sasbdb.org/media/fitting_files/log_files/feedback.log","software_version":null,"description":""}],"estimated_volume_method":null,"pddf_software":"ATSAS GNOM","pddf_software_version":null,"i0_calibration_standard":null,"description":"Small-angle X-ray scattering (SAXS) experiments were performed at the SIBYLS beamline 12.3.1 at the Advanced Light Source. This beamline has additional inline instrumentation and detectors coupled to a size exclusion column. Purified P-Rex2 DH/PH was concentrated to 2 mg/ml and then buffer exchanged into 20mM HEPES (pH 7), 200mM NaCl, 2mM DTT, and 1% glycerol SEC running buffer. The X-ray wavelength was set to 1.127 Å and the sample-to-detector distance to 2,100 mm, which gives scattering vectors (q) ranging from 0.01 Å-1 to 0.4 Å-1. The scattering vector is q = 4πsinθ/λ, where 2θ is the scattering angle. The SAXS flow cell was coupled to an Agilent 1260 Infinity HPLC system using a Shodex PROTEIN KW-802.5 SEC column equilibrated with the running buffer with a flow rate of 0.65 ml/min. For SAXS measurements, 2 sec X-ray exposures were collected continuously during a 25 min elution. All frames for analyses had one SAXS frame corresponding to the running buffer before the detection of a peak subtracted from each.","experiment_description":null,"tags":[],"intensity_unit":"arbitrary","experimental_mw":41.6,"experimental_mw_error":null,"guinier_i0_mw":null,"guinier_i0_mw_error":null,"porod_mw":43.4,"porod_mw_error":null,"pddf_i0":9.84,"pddf_i0_error":0.07,"guinier_i0":9.67557,"guinier_i0_error":null,"pddf_rg":2.694,"pddf_rg_error":0.016,"guinier_rg":2.612,"guinier_rg_error":0.381,"pddf_dmax":8.4,"pddf_dmax_error":null,"porod_volume":52.3,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":11,"guinier_point_last":76,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDZV5_datloglog_img.png","symmetry":null,"last_modified":"2026-07-23T20:42:10.836675+02:00","bragg_peak":[]}