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17 hits found for de Pereda

SASDBA4 – Plakin domain fragment of Human plectin (central region spectrin repeats: SR3-SR4-SR5-SR6 and SH3)

Plakin domain fragment of Human plectin encompassing spectrin repeats SR3-SR4-SR5-SR6 and SH3 experimental SAS data
DAMMIF model
Sample: Plakin domain fragment of Human plectin encompassing spectrin repeats SR3-SR4-SR5-SR6 and SH3 monomer, Homo sapiens protein
Buffer: 20 mM Sodium Phosphate 150 mM NaCl 5% glycerol 3 mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Nov 26
...ded Rod-like Shape. J Biol Chem 291(36):18643-62 (2016)
...de Pereda JM
RgGuinier 5.1 nm
Dmax 21.0 nm
VolumePorod 91 nm3

SASDBB4 – Plakin domain fragment of Human plectin (C-terminal region spectrin repeats: SR7-SR8-SR9)

Plakin domain fragment of Human plectin encompassing spectrin repeats SR7-SR8-SR9 experimental SAS data
DAMMIF model
Sample: Plakin domain fragment of Human plectin encompassing spectrin repeats SR7-SR8-SR9 monomer, Homo sapiens protein
Buffer: 20 mM Sodium Phosphate 150 mM NaCl 5% glycerol 3 mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Nov 26
...ded Rod-like Shape. J Biol Chem 291(36):18643-62 (2016)
...de Pereda JM
RgGuinier 4.2 nm
Dmax 17.0 nm
VolumePorod 57 nm3

SASDBC4 – Plakin domain of Human plectin (spectrin repeats: SR3-SR9)

Plakin domain fragment of Human plectin encompassing spectrin repeats SR3-SR9 experimental SAS data
DAMMIF model
Sample: Plakin domain fragment of Human plectin encompassing spectrin repeats SR3-SR9 monomer, Homo sapiens protein
Buffer: 20 mM Sodium Phosphate 150 mM NaCl 5% glycerol 3 mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Aug 13
...ded Rod-like Shape. J Biol Chem 291(36):18643-62 (2016)
...de Pereda JM
RgGuinier 8.5 nm
Dmax 35.0 nm
VolumePorod 135 nm3

SASDBD4 – ...desmoplakin (C-terminal region spectrin repeats: SR7-SR8-SR9)

Plakin domain fragment of Human Desmoplakin encompassing spectrin repeats SR7-SR8-SR9 experimental SAS data
DAMMIF model
Sample: ...Desmoplakin encompassing spectrin repeats SR7-SR8-SR9 monomer, Homo sapiens protein
Buffer: 20 mM Sodium Phosphate 150 mM NaCl 5% glycerol 3 mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2015 Sep 25
...ded Rod-like Shape. J Biol Chem 291(36):18643-62 (2016)
...de Pereda JM
RgGuinier 4.4 nm
Dmax 17.5 nm
VolumePorod 69 nm3

SASDBE4 – ...desmoplakin

Plakin domain of Human Desmoplakin experimental SAS data
Plakin domain of Human desmoplakin Rg histogram
Sample: ...Desmoplakin monomer, Homo sapiens protein
Buffer: 20 mM Sodium Phosphate 150 mM NaCl 5% glycerol 3 mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2015 Sep 25
...ded Rod-like Shape. J Biol Chem 291(36):18643-62 (2016)
...de Pereda JM
RgGuinier 7.5 nm
Dmax 35.0 nm
VolumePorod 136 nm3

SASDDF8 – ...des the final part of the connecting segment and the third and fourth FnIII domains (FnIII-3,4). Point mutant R1463E

Integrin beta-4 (1436-1666) R1463E experimental SAS data
Integrin beta4, fragment of the cytoplasmic region that includes the final part of the connecting segment and the third and fourth FnIII domains (FnIII-3,4). Point mutant R1463E Rg histogram
Sample: Integrin beta-4 (1436-1666) R1463E monomer, Homo sapiens protein
Buffer: 20 mM Sodium Phosphate 150 mM NaCl 5% glycerol 3 mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Nov 26
...desmosomes: Supplemental figures S1 to S10 and table S1 (2018)
...dez M, Carabias A, Alonso-García N, García-Rubio I, Kreft M, Sonnenberg A, de Pereda J
RgGuinier 2.3 nm
Dmax 9.5 nm
VolumePorod 35 nm3

...DE8 – ...des the final part of the connecting segment and the third and fourth FnIII domains (FnIII-3,4)

Integrin beta-4 fragment with final part of the connecting segment and the third and fourth FnIII domains experimental SAS data
Integrin beta4, fragment of the cytoplasmic region that includes the final part of the connecting segment and the third and fourth FnIII domains (FnIII-3,4) Rg histogram
Sample: Integrin beta-4 fragment with final part of the connecting segment and the third and fourth FnIII domains monomer, Homo sapiens protein
Buffer: 20 mM Sodium Phosphate 150 mM NaCl 5% glycerol 3 mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Nov 26
...desmosomes: Supplemental figures S1 to S10 and table S1 (2018)
...dez M, Carabias A, Alonso-García N, García-Rubio I, Kreft M, Sonnenberg A, de Pereda J
RgGuinier 2.4 nm
Dmax 9.5 nm
VolumePorod 37 nm3

SASDDH8 – ...des the final part of the connecting segment and the third and fourth FnIII domains (FnIII-3,4). Point mutant A1468R

Integrin beta-4 (1436-1666) A1468R experimental SAS data
Integrin beta4, fragment of the cytoplasmic region that includes the final part of the connecting segment and the third and fourth FnIII domains (FnIII-3,4). Point mutant A1468R Rg histogram
Sample: Integrin beta-4 (1436-1666) A1468R monomer, Homo sapiens protein
Buffer: 20 mM Sodium Phosphate 150 mM NaCl 5% glycerol 3 mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Nov 26
...desmosomes: Supplemental figures S1 to S10 and table S1 (2018)
...dez M, Carabias A, Alonso-García N, García-Rubio I, Kreft M, Sonnenberg A, de Pereda J
RgGuinier 2.8 nm
Dmax 10.9 nm
VolumePorod 35 nm3

SASDDJ8 – ...des the final part of the connecting segment and the third and fourth FnIII domains (FnIII-3,4). Point mutant A1468D

Integrin beta-4 (1436-1666) A1468D experimental SAS data
Integrin beta4, fragment of the cytoplasmic region that includes the final part of the connecting segment and the third and fourth FnIII domains (FnIII-3,4). Point mutant A1468D Rg histogram
Sample: Integrin beta-4 (1436-1666) A1468D monomer, Homo sapiens protein
Buffer: 20 mM Sodium Phosphate 150 mM NaCl 5% glycerol 3 mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Nov 26
...desmosomes: Supplemental figures S1 to S10 and table S1 (2018)
...dez M, Carabias A, Alonso-García N, García-Rubio I, Kreft M, Sonnenberg A, de Pereda J
RgGuinier 2.7 nm
Dmax 11.0 nm
VolumePorod 34 nm3

SASDDK8 – ...des the final part of the connecting segment and the third and fourth FnIII domains (FnIII-3,4). Point mutant R1475E

Integrin beta-4 (1436-1666) R1475E experimental SAS data
Integrin beta4, fragment of the cytoplasmic region that includes the final part of the connecting segment and the third and fourth FnIII domains (FnIII-3,4). Point mutant R1475E Rg histogram
Sample: Integrin beta-4 (1436-1666) R1475E monomer, Homo sapiens protein
Buffer: 20 mM Sodium Phosphate 150 mM NaCl 5% glycerol 3 mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Nov 26
...desmosomes: Supplemental figures S1 to S10 and table S1 (2018)
...dez M, Carabias A, Alonso-García N, García-Rubio I, Kreft M, Sonnenberg A, de Pereda J
RgGuinier 2.6 nm
Dmax 9.5 nm
VolumePorod 42 nm3

SASDDL8 – ...des the final part of the connecting segment and the third and fourth FnIII domains (FnIII-3,4). Point mutant R1475A

Integrin beta-4 (1436-1666) R1475A experimental SAS data
Integrin beta4, fragment of the cytoplasmic region that includes the final part of the connecting segment and the third and fourth FnIII domains (FnIII-3,4). Point mutant R1475A Rg histogram
Sample: Integrin beta-4 (1436-1666) R1475A monomer, Homo sapiens protein
Buffer: 20 mM Sodium Phosphate 150 mM NaCl 5% glycerol 3 mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Nov 26
...desmosomes: Supplemental figures S1 to S10 and table S1 (2018)
...dez M, Carabias A, Alonso-García N, García-Rubio I, Kreft M, Sonnenberg A, de Pereda J
RgGuinier 2.4 nm
Dmax 9.5 nm
VolumePorod 35 nm3

SASDDM8 – ...des the final part of the connecting segment and the third and fourth FnIII domains (FnIII-3,4). Point mutant R1542E

Integrin beta-4 (1436-1666) R1542E experimental SAS data
Integrin beta4, fragment of the cytoplasmic region that includes the final part of the connecting segment and the third and fourth FnIII domains (FnIII-3,4). Point mutant R1542E Rg histogram
Sample: Integrin beta-4 (1436-1666) R1542E monomer, Homo sapiens protein
Buffer: 20 mM Sodium Phosphate 150 mM NaCl 5% glycerol 3 mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Nov 26
...desmosomes: Supplemental figures S1 to S10 and table S1 (2018)
...dez M, Carabias A, Alonso-García N, García-Rubio I, Kreft M, Sonnenberg A, de Pereda J
RgGuinier 2.3 nm
Dmax 9.5 nm
VolumePorod 35 nm3

SASDDN8 – ...des the final part of the connecting segment and the third and fourth FnIII domains (FnIII-3,4). Point mutant R1542A

Integrin beta-4 (1436-1666) R1542A experimental SAS data
Integrin beta4, fragment of the cytoplasmic region that includes the final part of the connecting segment and the third and fourth FnIII domains (FnIII-3,4). Point mutant R1542A Rg histogram
Sample: Integrin beta-4 (1436-1666) R1542A monomer, Homo sapiens protein
Buffer: 20 mM Sodium Phosphate 150 mM NaCl 5% glycerol 3 mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Nov 26
...desmosomes: Supplemental figures S1 to S10 and table S1 (2018)
...dez M, Carabias A, Alonso-García N, García-Rubio I, Kreft M, Sonnenberg A, de Pereda J
RgGuinier 2.3 nm
Dmax 8.5 nm
VolumePorod 34 nm3

SASDDP8 – ...des the final part of the connecting segment and the third and fourth FnIII domains (FnIII-3,4). Point mutant T1663R

Integrin beta-4 (1436-1666) T1663R experimental SAS data
Integrin beta4, fragment of the cytoplasmic region that includes the final part of the connecting segment and the third and fourth FnIII domains (FnIII-3,4). Point mutant T1663R Rg histogram
Sample: Integrin beta-4 (1436-1666) T1663R monomer, Homo sapiens protein
Buffer: 20 mM Sodium Phosphate 150 mM NaCl 5% glycerol 3 mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Nov 26
...desmosomes: Supplemental figures S1 to S10 and table S1 (2018)
...dez M, Carabias A, Alonso-García N, García-Rubio I, Kreft M, Sonnenberg A, de Pereda J
RgGuinier 2.4 nm
Dmax 9.5 nm
VolumePorod 35 nm3

SASDAS6 – Plectin, fragment of the plakin domain encompassing the spectrin repeats SR3-SR4-SR5 and the SH3

Plectin experimental SAS data
DAMMIF model
Sample: Plectin monomer, Homo sapiens protein
Buffer: 20 mM Sodium Phosphate 150 mM NaCl 5% glycerol 2.5 mM DTT, pH: 7.5
Experiment: SAXS data collected at cSAXS, Swiss Light Source on 2009 Jul 24
The structure of the plakin domain of plectin reveals a non-canonical SH3 domain interacting with its fourth spectrin repeat. J Biol Chem 286(14):12429-38 (2011)
...de Pereda JM
RgGuinier 4.4 nm
Dmax 14.5 nm
VolumePorod 57 nm3

SASDAT6 – Integrin beta4, fragment of the cytoplasmic region encompassing the third and fourth FnIII domains

Integrin beta-4 experimental SAS data
DAMMIF model
Sample: Integrin beta-4 monomer, Homo sapiens protein
Buffer: 20 mM Sodium Phosphate 150 mM NaCl 5% glycerol 3 mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Aug 6
...DEER to obtain the structure of the FnIII-3,4 domains of integrin α6β4. Acta Crystallogr D Biol Crystallogr 71(Pt 4):969-85 (2015)
...de Pereda JM
RgGuinier 2.2 nm
Dmax 7.0 nm
VolumePorod 32 nm3

SASDDG8 – ...des the final part of the connecting segment and the third and fourth FnIII domains (FnIII-3,4). Point mutant R1463A

Integrin beta-4 (1436-1666) R1463A experimental SAS data
Integrin beta4, fragment of the cytoplasmic region that includes the final part of the connecting segment and the third and fourth FnIII domains (FnIII-3,4). Point mutant R1463A Rg histogram
Sample: Integrin beta-4 (1436-1666) R1463A monomer, Homo sapiens protein
Buffer: 20 mM Sodium Phosphate 150 mM NaCl 5% glycerol 3 mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Nov 26
...desmosomes: Supplemental figures S1 to S10 and table S1 (2018)
...dez M, Carabias A, Alonso-García N, García-Rubio I, Kreft M, Sonnenberg A, de Pereda J
RgGuinier 2.4 nm
Dmax 9.5 nm
VolumePorod 36 nm3