SASBDB entries for UniProt ID: Q15149

SASDAS6 – Plectin, fragment of the plakin domain encompassing the spectrin repeats SR3-SR4-SR5 and the SH3

UniProt ID: Q15149 (None-None) Plectin

Plectin experimental SAS data
DAMMIF model
Sample: Plectin monomer, 43 kDa Homo sapiens protein
Buffer: 20 mM Sodium Phosphate 150 mM NaCl 5% glycerol 2.5 mM DTT, pH: 7.5
Experiment: SAXS data collected at cSAXS, Swiss Light Source on 2009 Jul 24
The structure of the plakin domain of plectin reveals a non-canonical SH3 domain interacting with its fourth spectrin repeat. J Biol Chem 286(14):12429-38 (2011)
Ortega E, Buey RM, Sonnenberg A, de Pereda JM
RgGuinier 4.4 nm
Dmax 14.5 nm
VolumePorod 57 nm3

SASDBA4 – Plakin domain fragment of Human plectin (central region spectrin repeats: SR3-SR4-SR5-SR6 and SH3)

UniProt ID: Q15149-2 (543-1006) Plakin domain fragment of Human plectin encompassing spectrin repeats SR3-SR4-SR5-SR6 and SH3

Plakin domain fragment of Human plectin encompassing spectrin repeats SR3-SR4-SR5-SR6 and SH3 experimental SAS data
DAMMIF model
Sample: Plakin domain fragment of Human plectin encompassing spectrin repeats SR3-SR4-SR5-SR6 and SH3 monomer, 53 kDa Homo sapiens protein
Buffer: 20 mM Sodium Phosphate 150 mM NaCl 5% glycerol 3 mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Nov 26
The Structure of the Plakin Domain of Plectin Reveals an Extended Rod-like Shape. J Biol Chem 291(36):18643-62 (2016)
Ortega E, Manso JA, Buey RM, Carballido AM, Carabias A, Sonnenberg A, de Pereda JM
RgGuinier 5.1 nm
Dmax 21.0 nm
VolumePorod 91 nm3

SASDBB4 – Plakin domain fragment of Human plectin (C-terminal region spectrin repeats: SR7-SR8-SR9)

UniProt ID: Q15149-2 (1004-1372) Plakin domain fragment of Human plectin encompassing spectrin repeats SR7-SR8-SR9

Plakin domain fragment of Human plectin encompassing spectrin repeats SR7-SR8-SR9 experimental SAS data
DAMMIF model
Sample: Plakin domain fragment of Human plectin encompassing spectrin repeats SR7-SR8-SR9 monomer, 43 kDa Homo sapiens protein
Buffer: 20 mM Sodium Phosphate 150 mM NaCl 5% glycerol 3 mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Nov 26
The Structure of the Plakin Domain of Plectin Reveals an Extended Rod-like Shape. J Biol Chem 291(36):18643-62 (2016)
Ortega E, Manso JA, Buey RM, Carballido AM, Carabias A, Sonnenberg A, de Pereda JM
RgGuinier 4.2 nm
Dmax 17.0 nm
VolumePorod 57 nm3

SASDBC4 – Plakin domain of Human plectin (spectrin repeats: SR3-SR9)

UniProt ID: Q15149-2 (543-1372) Plakin domain fragment of Human plectin encompassing spectrin repeats SR3-SR9

Plakin domain fragment of Human plectin encompassing spectrin repeats SR3-SR9 experimental SAS data
DAMMIF model
Sample: Plakin domain fragment of Human plectin encompassing spectrin repeats SR3-SR9 monomer, 96 kDa Homo sapiens protein
Buffer: 20 mM Sodium Phosphate 150 mM NaCl 5% glycerol 3 mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Aug 13
The Structure of the Plakin Domain of Plectin Reveals an Extended Rod-like Shape. J Biol Chem 291(36):18643-62 (2016)
Ortega E, Manso JA, Buey RM, Carballido AM, Carabias A, Sonnenberg A, de Pereda JM
RgGuinier 8.5 nm
Dmax 35.0 nm
VolumePorod 135 nm3