Human alphacoronavirus non-structural protein Nsp10

Sven Falke, Al Kikhney.

SASDA39 – HCoV-229E Nsp10 in the absence of Zn

HCoV-229E Non-structural protein 10
MWexperimental 14 kDa
MWexpected 15 kDa
log I(s) 1.94×100 1.94×10-1 1.94×10-2 1.94×10-3
HCoV-229E Non-structural protein 10 small angle scattering data  s, nm-1
ln I(s)
HCoV-229E Non-structural protein 10 Guinier plot ln 1.94×100 Rg: 1.9 nm 0 (1.9 nm)-2 s2
(sRg)2I(s)/I(0)
HCoV-229E Non-structural protein 10 Kratky plot 1.104 0 √3 sRg
p(r)
HCoV-229E Non-structural protein 10 pair distance distribution function Rg: 2.0 nm 0 Dmax: 6.9 nm

Data validation

Fits and models

log I(s)
 s, nm-1
HCoV-229E Non-structural protein 10 CORAL model
log I(s)
 s, nm-1
HCoV-229E Non-structural protein 10 DAMMIF model
Synchrotron SAXS data from solutions of HCoV-229E Nsp10 in the absence of Zn in 25 mM HEPES 400 mM NaCl 1 mM EDTA 5% glycerol 40 mM NaH2PO4, pH 7.9 were collected on the EMBL P12 beam line at the PETRA III storage ring (DESY; Hamburg, Germany) using a Pilatus 2M detector at a sample-detector distance of 3.1 m and at a wavelength of λ = 0.12 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). Solute concentrations ranging between 0.5 and 2.8 mg/ml were measured at 10°C. Eight successive 5 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted. The low angle data collected at lower concentrations were extrapolated to infinite dilution and merged with the higher concentration data to yield the final composite scattering curve.

HCoV-229E Non-structural protein 10 (Nsp10)
Mol. type   Protein
Organism   Human coronavirus 229E
Olig. state   Monomer
Mon. MW   14.5 kDa
 
UniProt   P0C6U2 (3934-4068)
Sequence   FASTA