The BR domain of PsrP interacts with extracellular DNA to promote bacterial aggregation; structural insights into pneumococcal biofilm formation.

Schulte T, Mikaelsson C, Beaussart A, Kikhney A, Deshmukh M, Wolniak S, Pathak A, Ebel C, Löfling J, Fogolari F, Henriques-Normark B, Dufrêne YF, Svergun D, Nygren PÅ, Achour A, Sci Rep 6:32371 (2016) Europe PMC

SASDAC6 – PsrP functional binding region

Functional binding region (187-385) of the pneumococcal serine-rich repeat protein
MWI(0) 17 kDa
MWexpected 22 kDa
VPorod 38 nm3
log I(s) 1.85×101 1.85×100 1.85×10-1 1.85×10-2
Functional binding region (187-385) of the pneumococcal serine-rich repeat protein small angle scattering data  s, nm-1
ln I(s)
Functional binding region (187-385) of the pneumococcal serine-rich repeat protein Guinier plot ln 1.85×101 Rg: 2.0 nm 0 (2.0 nm)-2 s2
(sRg)2I(s)/I(0)
Functional binding region (187-385) of the pneumococcal serine-rich repeat protein Kratky plot 1.104 0 √3 sRg
p(r)
Functional binding region (187-385) of the pneumococcal serine-rich repeat protein pair distance distribution function Rg: 2.1 nm 0 Dmax: 7.8 nm

Data validation

Fits and models

log I(s)
 s, nm-1
PsrP functional binding region Rg histogram Rg, nm
Functional binding region (187-385) of the pneumococcal serine-rich repeat protein EOM/RANCH model
Functional binding region (187-385) of the pneumococcal serine-rich repeat protein EOM/RANCH model
Synchrotron SAXS data from solutions of PsrP functional binding region in PBS 5 % Glycerol, pH 7.4 were collected on the BM29 beam line at the ESRF storage ring (Grenoble, France) using a Pilatus 1M detector at a sample-detector distance of 2.4 m and at a wavelength of λ = 0.09919 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 2.90 mg/ml was measured at 20°C. 10 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Functional binding region (187-385) of the pneumococcal serine-rich repeat protein (PsrP BR(187-385))
Mol. type   Protein
Organism   Streptococcus pneumoniae
Olig. state   Monomer
Mon. MW   22.1 kDa
 
UniProt   A0A0H2URK1 (187-385)
Sequence   FASTA