| MWI(0) | 20 | kDa |
| MWexpected | 30 | kDa |
| VPorod | 41 | nm3 |
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log I(s)
2.18×101
2.18×100
2.18×10-1
2.18×10-2
|
s, nm-1
|
The BR domain of PsrP interacts with extracellular DNA to promote bacterial aggregation; structural insights into pneumococcal biofilm formation.
Schulte T, Mikaelsson C, Beaussart A, Kikhney A, Deshmukh M, Wolniak S, Pathak A, Ebel C, Löfling J, Fogolari F, Henriques-Normark B, Dufrêne YF, Svergun D, Nygren PÅ, Achour A, Sci Rep 6:32371 (2016) Europe PMCSASDAE6 – PsrP functional binding region
Functional binding region (120-395) of the pneumococcal serine-rich repeat protein|
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Fits and models
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Rg, nm
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Synchrotron SAXS
data from solutions of
PsrP functional binding region
in
PBS 5 % Glycerol, pH 7.4
were collected
on the
BM29 beam line
at the ESRF storage ring
(Grenoble, France)
using a Pilatus 1M detector
at a sample-detector distance of 2.4 m and
at a wavelength of λ = 0.93 nm
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
Solute concentrations ranging between 0.3 and 3 mg/ml were measured
at 20°C.
10 successive
1 second frames were collected.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
The low angle data collected at lower concentrations were extrapolated to infinite dilution and merged with the higher concentration data to yield the final composite scattering curve.
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