The modular structure of haemagglutinin/adhesin regions in gingipains of Porphyromonas gingivalis.

Li N, Yun P, Jeffries CM, Langley D, Gamsjaeger R, Church WB, Hunter N, Collyer CA, Mol Microbiol 81(5):1358-73 (2011) Europe PMC

SASDAF6 – K1K2K3 domain of Kgp gingipain

K1K2K3 adhesin modules of lysine-specific (Kgp) gingipain
MWexperimental 76 kDa
MWexpected 74 kDa
log I(s) 1.72×10-1 1.72×10-2 1.72×10-3 1.72×10-4
K1K2K3 adhesin modules of lysine-specific (Kgp) gingipain small angle scattering data  s, nm-1
ln I(s)
K1K2K3 adhesin modules of lysine-specific (Kgp) gingipain Guinier plot ln 1.72×10-1 Rg: 4.3 nm 0 (4.3 nm)-2 s2
(sRg)2I(s)/I(0)
K1K2K3 adhesin modules of lysine-specific (Kgp) gingipain Kratky plot 1.104 0 3 sRg
p(r)
K1K2K3 adhesin modules of lysine-specific (Kgp) gingipain pair distance distribution function Rg: 4.5 nm 0 Dmax: 14.7 nm

Data validation


Fits and models


log I(s)
 s, nm-1
K1K2K3 adhesin modules of lysine-specific (Kgp) gingipain BUNCH model

SAXS data from solutions of K1K2K3 domain of Kgp gingipain in 10 mM TRIS 150 mM NaCl, pH 7.6 were collected using an Anton Paar SAXSess 10 mm line collimated instrument (Sydney University, Australia) equipped with a Roper Scientific PI-SCX:4300 CCD detector at a sample-detector distance of 0.3 m and at a wavelength of λ = 0.1542 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 2.83 mg/ml was measured at 15°C. 1080 successive 10 second frames were collected. The data were normalized to the intensity of the transmitted beam and underwent 10 mm horizontal-width integration; the scattering of the solvent-blank was subtracted and the final data were desmeared.

The primary SAXS data displayed in this entry, and subsequent I(0), Rg and p(r) profile, take into account the beam-profile geometry correction (10 mm horizontal slit).

K1K2K3 adhesin modules of lysine-specific (Kgp) gingipain (K1K2K3)
Mol. type   Protein
Organism   Porphyromonas gingivalis W83
Olig. state   Monomer
Mon. MW   73.9 kDa
 
UniProt   Q51817 (982-1662)
Sequence   FASTA