KSHV but not MHV-68 LANA induces a strong bend upon binding to terminal repeat viral DNA.

Ponnusamy R, Petoukhov MV, Correia B, Custodio TF, Juillard F, Tan M, Pires de Miranda M, Carrondo MA, Simas JP, Kaye KM, Svergun DI, McVey CE, Nucleic Acids Res 43(20):10039-54 (2015) Europe PMC

SASDAP8 – kLBS1-2 DNA

kLBS1-2 DNA
MWI(0) 30 kDa
MWexpected 24 kDa
VPorod 50 nm3
log I(s) 1.14×102 1.14×101 1.14×100 1.14×10-1
kLBS1-2 DNA small angle scattering data  s, nm-1
ln I(s)
kLBS1-2 DNA Guinier plot ln 1.14×102 Rg: 4.0 nm 0 (4.0 nm)-2 s2
(sRg)2I(s)/I(0)
kLBS1-2 DNA Kratky plot 1.104 0 √3 sRg
p(r)
kLBS1-2 DNA pair distance distribution function Rg: 4.1 nm 0 Dmax: 16 nm

Data validation

Fits and models

log I(s)
 s, nm-1
kLBS1-2 DNA CRYSOL model
Synchrotron SAXS data from solutions of kLBS1-2 DNA in Tris, pH 7.6 were collected on the BM29 beam line at the ESRF storage ring (Grenoble, France) using a Pilatus 1M detector at a sample-detector distance of 2.4 m and at a wavelength of λ = 0.93 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 0.50 mg/ml was measured. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Study of oligomeric states of mLANA and kLANA and their complexes with DNA

kLBS1-2 DNA
Mol. type   DNA
Organism   unidentified herpesvirus
Olig. state   Monomer
Mon. MW   24 kDa