Global Disordering in Stereo-Specific Protein Association

Gupta A, Reinartz I, Spilotros A, Jonna V, Hofer A, Svergun D, Schug A, Wolf-Watz M, Biophysical Journal 112(3):33a (2017) DOI

SASDB65 – Tyrosine-protein phosphatase (YopH)/putative yopH targeting protein (SycH) heterotrimeric complex

SycH putative yopH targeting protein
Tyrosine-protein phosphatase YopH
MWexperimental 52 kDa
MWexpected 45 kDa
VPorod 89 nm3
log I(s) 7.44×104 7.44×103 7.44×102 7.44×101
SycH putative yopH targeting protein Tyrosine-protein phosphatase YopH small angle scattering data  s, nm-1
ln I(s)
SycH putative yopH targeting protein Tyrosine-protein phosphatase YopH Guinier plot ln 7.45×104 Rg: 3.0 nm 0 (3.0 nm)-2 s2
(sRg)2I(s)/I(0)
SycH putative yopH targeting protein Tyrosine-protein phosphatase YopH Kratky plot 1.104 0 √3 sRg
p(r)
SycH putative yopH targeting protein Tyrosine-protein phosphatase YopH pair distance distribution function Rg: 3.0 nm 0 Dmax: 12.5 nm

Data validation

Fits and models

log I(s)
 s, nm-1
SycH putative yopH targeting protein Tyrosine-protein phosphatase YopH CRYSOL model
X-ray synchrotron radiation scattering data from a solution of dimeric YopH protein in complex with the tyrosine-protein phosphatase, SycH, in 50 mM HEPES, 2 mM TCEP, pH 6.8 were collected on the P12 beam line of Petra-III (Hamburg, Germany) using a Pilatus 2M detector (I(s) vs s; s = 4π sin θ/λ, where 2θ is the scattering angle and λ=0.12 nm). One solute concentration of 1.00 mg/ml was measured using an exposure time of 1 s (recorded as 20 x 0.050 s frames). The data were normalized to the intensity of the transmitted beam and radially averaged and the scattering from the matched solvent-blank was subtracted. The data were scaled to protein concentration. The results presented here were obtained from a single concentration scattering curve.

SycH putative yopH targeting protein (SycH)
Mol. type   Protein
Organism   Yersinia pseudotuberculosis
Olig. state   Dimer
Mon. MW   15.8 kDa
 
UniProt   I3NIC8 (1-141)
Sequence   FASTA
 
Tyrosine-protein phosphatase YopH (YopH)
Mol. type   Protein
Organism   Yersinia pseudotuberculosis
Olig. state   Monomer
Mon. MW   14.0 kDa
 
UniProt   P08538 (1-129)
Sequence   FASTA