Solution structures of polcalcin Phl p 7 in three ligation states: Apo-, hemi-Mg2+-bound, and fully Ca2+-bound.

Henzl MT, Sirianni AG, Wycoff WG, Tan A, Tanner JJ, Proteins 81(2):300-15 (2013) Europe PMC

SASDDJ2 – Calcium-bound polcalcin Phl p 7

polcalcin Phl p 7

MW^experimental	9	kDa
MW^expected	9	kDa
V^Porod	14	nm³

log I(s) 5.25×10¹ 5.25×10⁰ 5.25×10^-1 5.25×10^-2

polcalcin Phl p 7 small angle scattering data

s, nm^-1

Log plot Log-Log plot

ln I(s)

ln 5.25×10¹ R_g: 1.3 nm 0 (1.3 nm)^-2 s²

(sR_g)²I(s)/I(0)

1.104 0 √3 sR_g

p(r)	R_g: 1.3 nm 0 D_max: 3.6 nm

Data validation

Fits and models

log I(s)	s, nm^-1
+3 Δ/σ -3	s, nm^-1

Synchrotron SAXS data from solutions of calcium-bound polcalcin Phl p 7 in 0.15 M NaCl, 0.025 M HEPES, 100 uM Ca2+, pH 7.4, were collected on the 12.3.1 SIBYLS beam line at the Advanced Light Source storage ring (ALS; Berkeley, CA, USA) using a MAR 165 CCD detector at a wavelength of λ = 0.1127 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). Solute concentrations ranging between 5 and 15 mg/ml were measured at 10°C. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Storage temperature = UNKNOWN. Sample detector distance = UNKNOWN. X-ray Exposure time = UNKNOWN. Number of frames = UNKNOWN

polcalcin Phl p 7
Mol. type		Protein
Organism		Phleum pratense
Olig. state		Monomer
Mon. MW		8.5 kDa

UniProt		O82040
Sequence		FASTA