The X-ray structure of human calbindin-D28K: an improved model.

Noble JW, Almalki R, Roe SM, Wagner A, Duman R, Atack JR, Acta Crystallogr D Struct Biol 74(Pt 10):1008-1014 (2018) Europe PMC

SASDDL6 – Calcium Loaded Calbindin-D28K

Calbindin
MWexperimental 25 kDa
MWexpected 30 kDa
VPorod 47 nm3
log I(s) 8.26×10-2 8.26×10-3 8.26×10-4 8.26×10-5
Calbindin small angle scattering data  s, nm-1
ln I(s)
Calbindin Guinier plot ln 8.26×10-2 Rg: 2.1 nm 0 (2.1 nm)-2 s2
(sRg)2I(s)/I(0)
Calbindin Kratky plot 1.104 0 √3 sRg
p(r)
Calbindin pair distance distribution function Rg: 2.2 nm 0 Dmax: 7.3 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Calbindin PDB (PROTEIN DATA BANK) model
log I(s)
 s, nm-1
Calbindin PDB (PROTEIN DATA BANK) model
Synchrotron SAXS data from solutions of calcium-loaded calbindin-D28K in 20 mM Tris, 150 mM NaCl, 3 mM CaCl2, pH 7.8, were collected on the B21 beam line at the Diamond Light Source (Oxfordshire, UK) using a Pilatus 2M detector at a sample-detector distance of 4 m and at a wavelength of λ = 0.1 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 11.50 mg/ml was measured at 25°C. 15 successive 3 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

SEC column = UNKNOWN. Sample injection volume = UNKNOWN. Flow rate = UNKNOWN

Calbindin (CALB1)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Monomer
Mon. MW   30.3 kDa
 
UniProt   P05937
Sequence   FASTA
 
PDB ID   6FIE
 
PDB ID   2G9B