The structure of SALM5 suggests a dimeric assembly for the presynaptic RPTP ligand recognition.

Karki S, Paudel P, Sele C, Shkumatov AV, Kajander T, Protein Eng Des Sel (2018) Europe PMC

SASDDZ2 – Leucine-rich repeat and fibronectin type-III domain-containing protein 4: Fragment SALM3 LRR-Ig-Fn

Leucine-rich repeat and fibronectin type-III domain-containing protein 4
MWexperimental 215 kDa
MWexpected 109 kDa
VPorod 313 nm3
log I(s) 4.21×101 4.21×100 4.21×10-1 4.21×10-2
Leucine-rich repeat and fibronectin type-III domain-containing protein 4 small angle scattering data  s, nm-1
ln I(s)
Leucine-rich repeat and fibronectin type-III domain-containing protein 4 Guinier plot ln 4.22×101 Rg: 4.8 nm 0 (4.8 nm)-2 s2
(sRg)2I(s)/I(0)
Leucine-rich repeat and fibronectin type-III domain-containing protein 4 Kratky plot 1.104 0 √3 sRg
p(r)
Leucine-rich repeat and fibronectin type-III domain-containing protein 4 pair distance distribution function Rg: 4.9 nm 0 Dmax: 17.1 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Leucine-rich repeat and fibronectin type-III domain-containing protein 4: Fragment SALM3 LRR-Ig-Fn Rg histogram Rg, nm
Leucine-rich repeat and fibronectin type-III domain-containing protein 4 EOM/RANCH model
Leucine-rich repeat and fibronectin type-III domain-containing protein 4 EOM/RANCH model
Leucine-rich repeat and fibronectin type-III domain-containing protein 4 EOM/RANCH model
Leucine-rich repeat and fibronectin type-III domain-containing protein 4 EOM/RANCH model
log I(s)
 s, nm-1
Leucine-rich repeat and fibronectin type-III domain-containing protein 4 DAMMIN model
Synchrotron SAXS data from solutions of Leucine-rich repeat and fibronectin type-III domain-containing protein 4 (fragment SALM3 LRR-Ig-Fn) in 20 mM Tris HCl, 100 mM NaCl, 0.02% NaN3, pH 7.5, were collected on the BM29 beam line at the ESRF storage ring (Grenoble, France) using a Pilatus 1M detector at a sample-detector distance of 2.9 m and at a wavelength of λ = 0.09919 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). A solute concentration of 23.3 mg/ml was injected onto a size exclusion chromatography column and the eluted sample and buffer were measured at 4.7°C. The data were normalized to the intensity of the transmitted beam and radially averaged and the scattering of an appropriate solvent-blank was subtracted to produce the SAXS data displayed in this entry.

SEC column = UNKNOWN. Sample injection volume = UNKNOWN. Flow rate = UNKNOWN

Leucine-rich repeat and fibronectin type-III domain-containing protein 4 (LRR-Ig-Fn)
Mol. type   Protein
Organism   Mus musculus
Olig. state   Dimer
Mon. MW   54.6 kDa
 
UniProt   Q80XU8 (17-510)
Sequence   FASTA