Increased association between Epstein-Barr virus EBNA2 from type 2 strains and the transcriptional repressor BS69 restricts B cell growth

Ponnusamy R, Khatri R, Correia P, Mancini E, Farrell P, West M, (2018) DOI

SASDEG6 – Epstein-Barr nuclear antigen 2 (EBNA2 Type2, amino acids 348-422)

Epstein-Barr nuclear antigen 2
MWexperimental 18 kDa
MWexpected 16 kDa
VPorod 20 nm3
log I(s) 2.68×10-2 2.68×10-3 2.68×10-4 2.68×10-5
Epstein-Barr nuclear antigen 2 small angle scattering data  s, nm-1
ln I(s)
Epstein-Barr nuclear antigen 2 Guinier plot ln 2.68×10-2 Rg: 2.8 nm 0 (2.8 nm)-2 s2
(sRg)2I(s)/I(0)
Epstein-Barr nuclear antigen 2 Kratky plot 1.104 0 3 sRg
p(r)
Epstein-Barr nuclear antigen 2 pair distance distribution function Rg: 3.0 nm 0 Dmax: 10.4 nm

Data validation


Fits and models


log I(s)
 s, nm-1
Epstein-Barr nuclear antigen 2 DAMMIN model
Epstein-Barr nuclear antigen 2 DAMMIF model

Synchrotron SAXS data from solutions of Epstein-Barr nuclear antigen 2 (EBNA2 Type2, amino acids 348-422) in 20mM Tris-HCl, 100mM NaCl, 2% Sucrose and 1mM TCEP, pH 7.5 were collected on the B21 beam line at the Diamond Light Source storage ring (Oxfordshire, UK) using a Pilatus 2M detector at a sample-detector distance of 4.0 m and at a wavelength of λ = 0.1 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). The sample was measured using size exclusion chromatography SAXS (SEC-SAXS) at 25°C. 100 successive 3 second frames were collected through the SEC sample elution peak. The data were normalized to the intensity of the transmitted beam and radially averaged and the scattering of an appropriate solvent-blank was subtracted.

SEC-SAXS was performed using the following parameters: Column type: S200 3.2/300 increase; Flow rate: 0.075 ml/min; Injection volume: 45 µl; Sample injection concentration: 4.6 mg/ml.

Tags: idp
Epstein-Barr nuclear antigen 2 (EBNA2 Type2)
Mol. type   Protein
Organism   Human gammaherpesvirus 4
Olig. state   Dimer
Mon. MW   7.9 kDa
 
UniProt   Q69022 (348-422)
Sequence   FASTA