Structural Organization and Dynamics of Homodimeric Cytohesin Family Arf GTPase Exchange Factors in Solution and on Membranes.

Das S, Malaby AW, Nawrotek A, Zhang W Zeghouf M, Maslen S, Skehel M, Chakravarthy S, Irving TC, Bilsel O, Cherfils J, Lambright DG, Structure (2019) Europe PMC

SASDEW9 – Cytohesin-2; ARF nucleotide-binding site opener, ARNO

Cytohesin-2 ARF nucleotide-binding site opener
MWexperimental 94 kDa
MWexpected 93 kDa
VPorod 145 nm3
log I(s) 4.39×101 4.39×100 4.39×10-1 4.39×10-2
Cytohesin-2 ARF nucleotide-binding site opener small angle scattering data  s, nm-1
ln I(s)
Cytohesin-2 ARF nucleotide-binding site opener Guinier plot ln 4.40×101 Rg: 4.8 nm 0 (4.8 nm)-2 s2
(sRg)2I(s)/I(0)
Cytohesin-2 ARF nucleotide-binding site opener Kratky plot 1.104 0 √3 sRg
p(r)
Cytohesin-2 ARF nucleotide-binding site opener pair distance distribution function Rg: 5.1 nm 0 Dmax: 19.7 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Cytohesin-2 ARF nucleotide-binding site opener GASBOR model
Synchrotron SAXS data from solutions of the cytohesin-2; ARF nucleotide-binding site opener, ARNO, in 300 mM NaCl, 2 mM 2-mercaptoethanol and 30 mM Tris-HCl, pH 7.5 were collected on the BM29 beam line at the ESRF (Grenoble, France) using a Dectris Pilatus 1M detector at a wavelength of λ = 0.993 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 10.00 mg/ml was measured at 15°C. 1500 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Sample detector distance = UNKNOWN

Cytohesin-2 ARF nucleotide-binding site opener (ARNO)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Dimer
Mon. MW   46.6 kDa
 
UniProt   Q99418 (1-400)
Sequence   FASTA