Interplay of Protein Disorder in Retinoic Acid Receptor Heterodimer and Its Corepressor Regulates Gene Expression.

Cordeiro TN, Sibille N, Germain P, Barthe P, Boulahtouf A, Allemand F, Bailly R, Vivat V, Ebel C, Barducci A, Bourguet W, le Maire A, Bernadó P, Structure (2019) Europe PMC

SASDF34 – Free Nuclear receptor CoRepressor NID (spanning from residue Gln2059 to Glu2325)

Nuclear receptor CoRepressor 1; Nuclear Receptor Interaction Domain (NID)
MWI(0) 26 kDa
MWexpected 29 kDa
VPorod 102 nm3
log I(s) 2.66×101 2.66×100 2.66×10-1 2.66×10-2
Nuclear receptor CoRepressor 1; Nuclear Receptor Interaction Domain (NID) small angle scattering data  s, nm-1
ln I(s)
Nuclear receptor CoRepressor 1; Nuclear Receptor Interaction Domain (NID) Guinier plot ln 2.66×101 Rg: 4.7 nm 0 (4.7 nm)-2 s2
(sRg)2I(s)/I(0)
Nuclear receptor CoRepressor 1; Nuclear Receptor Interaction Domain (NID) Kratky plot 1.104 0 √3 sRg
p(r)
Nuclear receptor CoRepressor 1; Nuclear Receptor Interaction Domain (NID) pair distance distribution function Rg: 5.0 nm 0 Dmax: 17.7 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Synchrotron SAXS data from solutions of Free Nuclear receptor CoRepressor NID (spanning from residue Gln2059 to Glu2325) in 50mM Tris HCl, 150mM NaCl, 2mM TCEP, pH 7.5 were collected on the BM29 beam line at the ESRF (Grenoble, France) using a Pilatus 1M detector at a sample-detector distance of 2.9 m and at a wavelength of λ = 0.099 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 2.00 mg/ml was measured at 20°C. 15 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Several datasets were collected at multiple concentrations ranging between 1.3 and 2.0 mg/ml. Only the data of 2mg/ml is shown.

Tags: idp
Nuclear receptor CoRepressor 1; Nuclear Receptor Interaction Domain (NID) (N-CoR-NID)
Mol. type   Protein
Organism   Mus musculus
Olig. state   Monomer
Mon. MW   29.1 kDa
 
UniProt   Q60974 (2059-2325)
Sequence   FASTA