Structural and mechanistic insights into the interaction of the circadian transcription factor BMAL1 with the KIX domain of the CREB-binding protein.

Garg A, Orru R, Ye W, Distler U, Chojnacki JE, Köhn M, Tenzer S, Sönnichsen C, Wolf E, J Biol Chem (2019) Europe PMC

SASDF37 – KIX domain (kinase-inducible domain interacting domain) of CREB-binding protein (Mus musculus), mutation L664C

Kinase-inducible domain interacting (KIX) domain of CREB-binding protein (CBP), mutation L664C
MWexperimental 13 kDa
MWexpected 10 kDa
log I(s) 3.36×103 3.36×102 3.36×101 3.36×100
Kinase-inducible domain interacting (KIX) domain of CREB-binding protein (CBP), mutation L664C small angle scattering data  s, nm-1
ln I(s)
Kinase-inducible domain interacting (KIX) domain of CREB-binding protein (CBP), mutation L664C Guinier plot ln 3.37×103 Rg: 1.9 nm 0 (1.9 nm)-2 s2
(sRg)2I(s)/I(0)
Kinase-inducible domain interacting (KIX) domain of CREB-binding protein (CBP), mutation L664C Kratky plot 1.104 0 √3 sRg
p(r)
Kinase-inducible domain interacting (KIX) domain of CREB-binding protein (CBP), mutation L664C pair distance distribution function Rg: 2.0 nm 0 Dmax: 8.1 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Kinase-inducible domain interacting (KIX) domain of CREB-binding protein (CBP), mutation L664C DAMMIN model
Synchrotron SAXS data from solutions of KIX domain (kinase-inducible domain interacting domain) of CREB-binding protein (Mus musculus), mutation L664C in 25 mM Hepes, 150 NaCl, 1 mM DTT, 5% Glycerol, pH 7.2 were collected on the EMBL P12 beam line at the PETRA III storage ring (DESY; Hamburg, Germany) using a Pilatus 2M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.124 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 5.72 mg/ml was measured at 10.1°C. 30 successive 0.045 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Kinase-inducible domain interacting (KIX) domain of CREB-binding protein (CBP), mutation L664C (CBP KIX domain)
Mol. type   Protein
Organism   Mus musculus
Olig. state   Monomer
Mon. MW   10.5 kDa
 
UniProt   P45481 (586-672)
Sequence   FASTA