Tetramerization at low pH licenses DNA methylation activity of M.HpyAXI in the presence of acid stress.

Narayanan N Banerjee A, Jain D, Kulkarni DS, Sharma R, Nirwal S, Rao DN, Nair DT, J Mol Biol (2019) Europe PMC

SASDFL6 – Adenine specific DNA methyltransferase (Mod Hp_0593) at pH 8

Adenine specific DNA methyltransferase (Mod)
MWexperimental 120 kDa
MWexpected 137 kDa
VPorod 143 nm3
log I(s) 3.55×101 3.55×100 3.55×10-1 3.55×10-2
Adenine specific DNA methyltransferase (Mod) small angle scattering data  s, nm-1
ln I(s)
Adenine specific DNA methyltransferase (Mod) Guinier plot ln 3.56×101 Rg: 3.3 nm 0 (3.3 nm)-2 s2
(sRg)2I(s)/I(0)
Adenine specific DNA methyltransferase (Mod) Kratky plot 1.104 0 √3 sRg
p(r)
Adenine specific DNA methyltransferase (Mod) pair distance distribution function Rg: 3.4 nm 0 Dmax: 12.5 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Adenine specific DNA methyltransferase (Mod) PYMOL model
log I(s)
 s, nm-1
Adenine specific DNA methyltransferase (Mod) PYMOL model
Synchrotron SAXS data from solutions of adenine specific DNA methyltransferase (Mod Hp_0593) at pH 8 in 25 mM Tris, 250 mM NaCl were collected on the ID14-3 beam line at the ESRF storage ring (Grenoble, France) using a Pilatus 1M detector at a wavelength of λ = 0.0992 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 2.00 mg/ml was measured at 10°C. 10 successive 0.500 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Sample detector distance = UNKNOWN

Adenine specific DNA methyltransferase (Mod)
Mol. type   Protein
Organism   Helicobacter pylori
Olig. state   Dimer
Mon. MW   68.3 kDa
 
UniProt   O25315 (1-598)
Sequence   FASTA