Tetramerization at low pH licenses DNA methylation activity of M.HpyAXI in the presence of acid stress.

Narayanan N Banerjee A, Jain D, Kulkarni DS, Sharma R, Nirwal S, Rao DN, Nair DT, J Mol Biol (2019) Europe PMC

SASDFM6 – Adenine specific DNA methyltransferase (Mod HP_0593) at pH 5.5

Adenine specific DNA methyltransferase (Mod)

MW^experimental	250	kDa
MW^expected	273	kDa
V^Porod	316	nm³

log I(s) 1.26×10² 1.26×10¹ 1.26×10⁰ 1.26×10^-1

Adenine specific DNA methyltransferase (Mod) small angle scattering data

s, nm^-1

Log plot Log-Log plot

ln I(s)

Adenine specific DNA methyltransferase (Mod) Guinier plot

ln 1.27×10² R_g: 5.0 nm 0 (5.0 nm)^-2 s²

(sR_g)²I(s)/I(0)

Adenine specific DNA methyltransferase (Mod) Kratky plot

1.104 0 √3 sR_g

p(r)	R_g: 4.9 nm 0 D_max: 19.1 nm

Data validation

Experimental data range

p(r) fit to data

Metric

Value

s_min D_max / π

0.7

N_Shannon

Worse

Better

Metric

Value

p_cormap

χ²

0.255
0.894

Worse

Better

Fits and models

log I(s)	s, nm^-1
+3 Δ/σ -3	s, nm^-1

Adenine specific DNA methyltransferase (Mod) PYMOL model

Synchrotron SAXS data from solutions of adenine specific DNA methyltransferase (Mod HP_0593) at pH 5.5 in 25 mM citrate, 250 mM NaCl were collected on the BM29 beam line at the ESRF storage ring (Grenoble, France) using a Pilatus 1M detector (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 2.00 mg/ml was measured. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

The sample is severely aggregated. Wavelength UNKNOWN; Experimental temperature, UNKNOWN; Exposure time, UNKNOWN; Sample to detector distance, UNKNOWN.

Adenine specific DNA methyltransferase (Mod)
Mol. type		Protein
Organism		Helicobacter pylori
Olig. state		Tetramer
Mon. MW		68.4 kDa

UniProt		O25315 (1-598)
Sequence		FASTA