Apolipoprotein E project

Lucas Kraft.

SASDGC9 – Apolipoprotein E3 (SEC-SAXS)

Apolipoprotein E3
MWexperimental 171 kDa
MWexpected 139 kDa
log I(s) 2.40×10-1 2.40×10-2 2.40×10-3 2.40×10-4
Apolipoprotein E3 small angle scattering data  s, nm-1
ln I(s)
Apolipoprotein E3 Guinier plot ln 2.40×10-1 Rg: 5.8 nm 0 (5.8 nm)-2 s2
(sRg)2I(s)/I(0)
Apolipoprotein E3 Kratky plot 1.104 0 √3 sRg
p(r)
Apolipoprotein E3 pair distance distribution function Rg: 6.0 nm 0 Dmax: 20 nm

Data validation

There are no models related to this curve.

Synchrotron SAXS data from solutions of Apolipoprotein E3 (SEC-SAXS) in 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH 8 were collected on the B21 beam line at the Diamond Light Source storage ring (Didcot, UK) using a Pilatus 2M detector at a sample-detector distance of 4.0 m and at a wavelength of λ = 0.1 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 45.00 μl sample at 10 mg/ml was injected at a 0.16 ml/min flow rate onto a Shodex KW403-4F column at 20°C. 13 successive 3 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

ApoE3 at 10 mg/mL was gel-filtered in 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH 8.0.

Apolipoprotein E3 (ApoE3)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Tetramer
Mon. MW   34.7 kDa
 
UniProt   P02649 (19-317)
Sequence   FASTA