The internal interaction in RBBP5 regulates assembly and activity of MLL1 methyltransferase complex.

Han J, Li T, Li Y, Li M, Wang X, Peng C, Su C, Li N, Li Y, Xu Y, Chen Y, Nucleic Acids Res (2019) Europe PMC

SASDGD4 – Mixed lineage leukemia protein-1 complex, MLL1-WDR5-ASH2L-RBBP5(2-381)

Retinoblastoma-binding protein 5
Histone-lysine N-methyltransferase 2A
WD repeat-containing protein 5
Set1/Ash2 histone methyltransferase complex subunit ASH2
MWexperimental 170 kDa
MWexpected 162 kDa
VPorod 360 nm3
log I(s) 6.86×10-2 6.86×10-3 6.86×10-4 6.86×10-5
Retinoblastoma-binding protein 5 Histone-lysine N-methyltransferase 2A WD repeat-containing protein 5 Set1/Ash2 histone methyltransferase complex subunit ASH2 small angle scattering data  s, nm-1
ln I(s)
Retinoblastoma-binding protein 5 Histone-lysine N-methyltransferase 2A WD repeat-containing protein 5 Set1/Ash2 histone methyltransferase complex subunit ASH2 Guinier plot ln 6.86×10-2 Rg: 5.7 nm 0 (5.7 nm)-2 s2
(sRg)2I(s)/I(0)
Retinoblastoma-binding protein 5 Histone-lysine N-methyltransferase 2A WD repeat-containing protein 5 Set1/Ash2 histone methyltransferase complex subunit ASH2 Kratky plot 1.104 0 3 sRg
p(r)
Retinoblastoma-binding protein 5 Histone-lysine N-methyltransferase 2A WD repeat-containing protein 5 Set1/Ash2 histone methyltransferase complex subunit ASH2 pair distance distribution function Rg: 5.7 nm 0 Dmax: 18.6 nm

Data validation


There are no models related to this curve.

Synchrotron SAXS data from solutions of MLL1-WDR5-ASH2L-RBBP5(2-381) in 300 mM NaCl, 25mM Tris-HCl, 4% glycerol, 1 mM TCEP, pH 8 were collected on the BL19U2 beam line at the Shanghai Synchrotron Radiation Facility (SSRF; Shanghai, China) using a Pilatus 1M detector at a sample-detector distance of 2.4 m and at a wavelength of λ = 0.0918 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 2.00 mg/ml was measured at 10°C. 20 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Retinoblastoma-binding protein 5 (RBBP5)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Monomer
Mon. MW   42.4 kDa
 
UniProt   Q15291 (2-381)
Sequence   FASTA
 
Histone-lysine N-methyltransferase 2A (MLL1)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Monomer
Mon. MW   24.9 kDa
 
UniProt   Q03164 (3754-3969)
Sequence   FASTA
 
WD repeat-containing protein 5 (WDR5)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Monomer
Mon. MW   34.3 kDa
 
UniProt   P61964 (23-334)
Sequence   FASTA
 
Set1/Ash2 histone methyltransferase complex subunit ASH2 (ASH2L)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Monomer
Mon. MW   60.2 kDa
 
UniProt   Q9UBL3-3 (1-534)
Sequence   FASTA