The internal interaction in RBBP5 regulates assembly and activity of MLL1 methyltransferase complex.

Han J, Li T, Li Y, Li M, Wang X, Peng C, Su C, Li N, Li Y, Xu Y, Chen Y, Nucleic Acids Res (2019) Europe PMC

SASDGF4 – Mixed lineage leukemia protein-1 complex, MLL1-WDR5-ASH2L-RBBP5(2-480)L399A/L400A/I457A/L459A

Histone-lysine N-methyltransferase 2A
WD repeat-containing protein 5
Set1/Ash2 histone methyltransferase complex subunit ASH2
Retinoblastoma-binding protein 5
MWexperimental 163 kDa
MWexpected 172 kDa
VPorod 313 nm3
log I(s) 5.78×10-2 5.78×10-3 5.78×10-4 5.78×10-5
Histone-lysine N-methyltransferase 2A WD repeat-containing protein 5 Set1/Ash2 histone methyltransferase complex subunit ASH2 Retinoblastoma-binding protein 5 small angle scattering data  s, nm-1
ln I(s)
Histone-lysine N-methyltransferase 2A WD repeat-containing protein 5 Set1/Ash2 histone methyltransferase complex subunit ASH2 Retinoblastoma-binding protein 5 Guinier plot ln 5.79×10-2 Rg: 5.3 nm 0 (5.3 nm)-2 s2
(sRg)2I(s)/I(0)
Histone-lysine N-methyltransferase 2A WD repeat-containing protein 5 Set1/Ash2 histone methyltransferase complex subunit ASH2 Retinoblastoma-binding protein 5 Kratky plot 1.104 0 3 sRg
p(r)
Histone-lysine N-methyltransferase 2A WD repeat-containing protein 5 Set1/Ash2 histone methyltransferase complex subunit ASH2 Retinoblastoma-binding protein 5 pair distance distribution function Rg: 5.4 nm 0 Dmax: 17.2 nm

Data validation


There are no models related to this curve.

Synchrotron SAXS data from solutions of MLL1-WDR5-ASH2L-RBBP5(2-480)L399A/L400A/I457A/L459A in 300 mM NaCl, 25mM Tris-HCl, 4% glycerol, 1 mM TCEP, pH 8 were collected on the BL19U2 beam line at the Shanghai Synchrotron Radiation Facility (SSRF; Shanghai, China) using a Pilatus 1M detector at a sample-detector distance of 2.4 m and at a wavelength of λ = 0.0918 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 2.00 mg/ml was measured at 10°C. 20 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Histone-lysine N-methyltransferase 2A (MLL1)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Monomer
Mon. MW   24.9 kDa
 
UniProt   Q03164 (3754-3969)
Sequence   FASTA
 
WD repeat-containing protein 5 (WDR5)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Monomer
Mon. MW   34.3 kDa
 
UniProt   P61964 (23-334)
Sequence   FASTA
 
Set1/Ash2 histone methyltransferase complex subunit ASH2 (ASH2L)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Monomer
Mon. MW   60.2 kDa
 
UniProt   Q9UBL3-3 (1-534)
Sequence   FASTA
 
Retinoblastoma-binding protein 5 (RBBP5)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Monomer
Mon. MW   52.8 kDa
 
UniProt   Q15291 (2-480)
Sequence   FASTA