Suppressor of Fused

Valerie Biou.

SASDGW5 – Suppressor of Fused from Drosophila

Suppressor of fused homolog
MWexperimental 51 kDa
MWexpected 53 kDa
VPorod 12 nm3
log I(s) 1.74×100 1.74×10-1 1.74×10-2 1.74×10-3
Suppressor of fused homolog small angle scattering data  s, nm-1
ln I(s)
Suppressor of fused homolog Guinier plot ln 1.74×100 Rg: 2.7 nm 0 (2.7 nm)-2 s2
(sRg)2I(s)/I(0)
Suppressor of fused homolog Kratky plot 1.104 0 √3 sRg
p(r)
Suppressor of fused homolog pair distance distribution function Rg: 2.7 nm 0 Dmax: 8.8 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Suppressor of fused homolog CUSTOM IN-HOUSE model
log I(s)
 s, nm-1
Suppressor of fused homolog CUSTOM IN-HOUSE model
Synchrotron SAXS data from solutions of suppressor of fused from Drosophila in 50 mM bis-TRIS pH 5.5, 200 mM NaCl, 10% glycerol, were collected on the SWING beam line at SOLEIL (Saint-Aubin, France) using a AVIEX PCCD170170 detector at a sample-detector distance of 1.8 m and at a wavelength of λ = 0.1 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 40.00 μl sample at 10 mg/ml was injected at a 0.30 ml/min flow rate onto a Agilent Bio SEC-3, 300 Å column at 10°C. 160 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Suppressor of fused homolog (dSuFu)
Mol. type   Protein
Organism   Drosophila melanogaster
Olig. state   Monomer
Mon. MW   52.8 kDa
 
UniProt   Q9VG38 (1-468)
Sequence   FASTA
 
PDB ID   4KMA
 
PDB ID   4KMA