Suppressor of Fused

Valerie Biou.

SASDGZ5 – Suppressor of Fused from Human D30

Suppressor of fused homolog
MWexperimental 51 kDa
MWexpected 51 kDa
log I(s) 2.25×100 2.25×10-1 2.25×10-2 2.25×10-3
Suppressor of fused homolog small angle scattering data  s, nm-1
ln I(s)
Suppressor of fused homolog Guinier plot ln 2.25×100 Rg: 3.2 nm 0 (3.2 nm)-2 s2
(sRg)2I(s)/I(0)
Suppressor of fused homolog Kratky plot 1.104 0 √3 sRg
p(r)
Suppressor of fused homolog pair distance distribution function Rg: 3.5 nm 0 Dmax: 14 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Suppressor of fused homolog CUSTOM IN-HOUSE model
Synchrotron SAXS data from solutions of suppressor of fused from Human D30 in 50 mM bis-TRIS pH 5.5, 200 mM NaCl, 10% glycerol, were collected on the SWING beam line at SOLEIL (Saint-Aubin, France) using a Eiger 4M detector at a sample-detector distance of 2.1 m and at a wavelength of λ = 0.1 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 40.00 μl sample at 14 mg/ml was injected at a 0.30 ml/min flow rate onto a Agilent Bio SEC-3, 300 Å column at 10°C. 200 successive 0.990 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Suppressor of fused homolog (hSuFuD30)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Monomer
Mon. MW   51.1 kDa
 
UniProt   Q9UMX1
Sequence   FASTA
 
PDB ID   4KM9