| MWexperimental | 16 | kDa |
| MWexpected | 20 | kDa |
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log I(s)
1.45×101
1.45×100
1.45×10-1
1.45×10-2
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s, nm-1
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Structural and Biophysical Analysis of the CLCA1 VWA Domain Suggests Mode of TMEM16A Engagement.
Berry KN, Brett TJ, Cell Rep 30(4):1141-1151.e3 (2020) Europe PMCSASDH24 – Calcium-Activated Chloride Channel Regulator 1 VWA domain
Calcium-activated chloride channel regulator 1|
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Fits and models
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Synchrotron SAXS
data from solutions of
Calcium-Activated Chloride Channel Regulator 1 VWA domain
in
20 mM HEPES, 150 mM NaCl, 2% glycerol, pH 7.4
were collected
on the
12-ID-B SAXS/WAXS beam line
at the Advanced Photon Source (APS), Argonne National Laboratory storage ring
(Lemont, IL, USA)
using a Pilatus 2M detector
at a sample-detector distance of 1.5 m and
at a wavelength of λ = 0.127 nm
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
Solute concentrations ranging between 2.0 and 8.2 mg/ml were measured
at 10°C.
32 successive
0.300 second frames were collected.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
The low angle data collected at lower concentration were merged with the highest concentration high angle data to yield the final composite scattering curve.
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