Improving data quality and expanding BioSAXS experiments to low-molecular-weight and low-concentration protein samples.

Castellví A, Pascual-Izarra C, Crosas E, Malfois M, Juanhuix J, Acta Crystallogr D Struct Biol 76(Pt 10):971-981 (2020) Europe PMC

SASDJG3 – Bovine Liver Catalase in presence of 100 mM 5-methyl uridine

Catalase
MWexperimental 250 kDa
MWexpected 240 kDa
VPorod 265 nm3
log I(s) 2.79×10-2 2.79×10-3 2.79×10-4 2.79×10-5
Catalase small angle scattering data  s, nm-1
ln I(s)
Catalase Guinier plot ln 2.79×10-2 Rg: 3.7 nm 0 (3.7 nm)-2 s2
(sRg)2I(s)/I(0)
Catalase Kratky plot 1.104 0 √3 sRg
Dmax: 11.5 nm

Data validation

There are no models related to this curve.

Synchrotron SAXS data from a solution of 6.69 mg/ml Bovine Liver Catalase in 50 mM potassium phosphate pH 7 was collected on the BL11-NCD-SWEET beam line at ALBA Synchrotron using a Pilatus 1M photon-counting detector. The beam at sample position was 0.566 mm (horizontal) × 0.420 mm (vertical), the incident photon flux was 1.08×10e12 photons/s at 12.4 keV (λ =0.0999 nm) and the sample to detector distance was 2.460 m. 2000 successive 0.1 second frames were collected and data were normalized to the intensity of the transmitted beam and radially averaged. The current buffer-subtracted scattering profile corresponds to the average of 1000 collected frames. The absorbed dose by the sample was more than 87.00 kGy.

Sample temperature: 25°C. CAUTION! The scattering intensities do not have associated errors.

Catalase (CAT)
Mol. type   Protein
Organism   Bos taurus
Olig. state   Tetramer
Mon. MW   59.9 kDa
 
UniProt   P00432 (1-527)
Sequence   FASTA