Wide-Angle X-ray Scattering (WAXS) Analysis of Small Nucleic Acids

Yen-Lin Chen.

SASDKG5 – 25 Based-Paired DNA Duplex

25 base-paired DNA Duplex
MWexperimental 15 kDa
MWexpected 15 kDa
log I(s) 8.44×100 8.44×10-1 8.44×10-2 8.44×10-3
25 base-paired DNA Duplex small angle scattering data  s, nm-1
ln I(s)
25 base-paired DNA Duplex Guinier plot ln 8.44×100 Rg: 2.3 nm 0 (2.3 nm)-2 s2
(sRg)2I(s)/I(0)
25 base-paired DNA Duplex Kratky plot 1.104 0 3 sRg
p(r)
25 base-paired DNA Duplex pair distance distribution function Rg: 2.4 nm 0 Dmax: 8.4 nm

Data validation


There are no models related to this curve.

Synchrotron SAXS data from solutions of 25 Based-Paired DNA Duplex in 100 mM NaCl, 10 mM sodium 3-(N-morpholino)propanesulfonic acid (Na-MOPS) and 20 uM EDTA, pH 7 were collected on the 16-ID (LiX) beam line at the National Synchrotron Light Source II (NSLS-II) storage ring (Upton, NY, USA) using a Pilatus3 S 1M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.092 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 2.75 mg/ml was measured at 10°C. Five successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

25 base-paired DNA Duplex (DNA)
Mol. type   DNA
Olig. state   Monomer
Mon. MW   15.5 kDa
Sequence   FASTA