Assembly of higher-order SMN oligomers is essential for metazoan viability and requires an exposed structural motif present in the YG zipper dimer.

Gupta K, Wen Y, Ninan NS, Raimer AC, Sharp R, Spring AM, Sarachan KL, Johnson MC, Van Duyne GD, Matera AG, Nucleic Acids Res 49(13):7644-7664 (2021) Europe PMC

SASDL65 – Schizosaccharomyces pombe SMN(GCN4IL) Chimera•Gemin2 complex (3 mg/mL)

Survival motor neuron-like protein 1
Survival of motor neuron protein-interacting protein yip11
Gcn4p
MWexperimental 121 kDa
MWexpected 89 kDa
VPorod 315 nm3
log I(s) 9.29×101 9.29×100 9.29×10-1 9.29×10-2
Survival motor neuron-like protein 1 Survival of motor neuron protein-interacting protein yip11 Gcn4p small angle scattering data  s, nm-1
ln I(s)
Survival motor neuron-like protein 1 Survival of motor neuron protein-interacting protein yip11 Gcn4p Guinier plot ln 9.30×101 Rg: 6.3 nm 0 (6.3 nm)-2 s2
(sRg)2I(s)/I(0)
Survival motor neuron-like protein 1 Survival of motor neuron protein-interacting protein yip11 Gcn4p Kratky plot 1.104 0 √3 sRg
p(r)
Survival motor neuron-like protein 1 Survival of motor neuron protein-interacting protein yip11 Gcn4p pair distance distribution function Rg: 6.6 nm 0 Dmax: 19.9 nm

Data validation

There are no models related to this curve.

Synchrotron SAXS data from solutions of the survival motor neuron SMN(GCN4IL) Chimera•Gemin2 complex in 20 mM Na/KPO4 pH 7.0, 150 mM NaCl, 1 mM DTT, were collected on the X21 beam line at the NSLS (Upton, USA) using a MAR165 detector at a sample-detector distance of 0.8 m and at a wavelength of λ = 0.1239 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 3.00 mg/ml was measured at 4°C. Three successive 30 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Survival motor neuron-like protein 1 (spSMN)
Mol. type   Protein
Organism   Schizosaccharomyces pombe (strain 972 / ATCC 24843)
Olig. state   Dimer
Mon. MW   13.2 kDa
 
UniProt   Q09808 (1-117)
Sequence   FASTA
 
Survival of motor neuron protein-interacting protein yip11 (spGemin2)
Mol. type   Protein
Organism   Schizosaccharomyces pombe (strain 972 / ATCC 24843)
Olig. state   Dimer
Mon. MW   27.0 kDa
 
UniProt   P0CU08 (1-235)
Sequence   FASTA
 
Gcn4p (GCN4(IL))
Mol. type   Protein
Organism   Saccharomyces cerevisiae (strain JAY291)
Olig. state   Dimer
Mon. MW   4.1 kDa
Sequence   FASTA