The Disease Associated Tau35 Fragment has an Increased Propensity to Aggregate Compared to Full-Length Tau

Lyu C, Da Vela S, Al-Hilaly Y, Marshall K, Thorogate R, Svergun D, Serpell L, Pastore A, Hanger D, Frontiers in Molecular Biosciences 8 (2021) DOI

SASDLT4 – Tau protein, 2N3R isoform

Microtubule-associated protein tau, 2N3R isoform
MWI(0) 37 kDa
MWexpected 43 kDa
VPorod 211 nm3
log I(s) 1.79×10-2 1.79×10-3 1.79×10-4 1.79×10-5
Microtubule-associated protein tau, 2N3R isoform small angle scattering data  s, nm-1
ln I(s)
Microtubule-associated protein tau, 2N3R isoform Guinier plot ln 1.80×10-2 Rg: 6.3 nm 0 (6.3 nm)-2 s2
(sRg)2I(s)/I(0)
Microtubule-associated protein tau, 2N3R isoform Kratky plot 1.104 0 √3 sRg
p(r)
Microtubule-associated protein tau, 2N3R isoform pair distance distribution function Rg: 6.3 nm 0 Dmax: 19.8 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Tau protein, 2N3R isoform Rg histogram Rg, nm
Synchrotron SAXS data from solutions of the 2N3R isoform of Tau protein in phosphate buffered saline, pH 7.4 were collected on the EMBL P12 beam line at PETRA III (DESY, Hamburg, Germany) using a Pilatus 6M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.124 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 100.00 μl sample at 8 mg/ml was injected at a 0.50 ml/min flow rate onto a GE Superdex 200 Increase 10/300 column at 20°C. 3000 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Tags: idp
Microtubule-associated protein tau, 2N3R isoform (Tau410, 2N3R isoform)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Monomer
Mon. MW   42.6 kDa
 
UniProt   P10636
Sequence   FASTA