The Cytoplasmic Tail of Influenza A Virus Hemagglutinin and Membrane Lipid Composition Change the Mode of M1 Protein Association with the Lipid Bilayer

Kordyukova L, Konarev P, Fedorova N, Shtykova E, Ksenofontov A, Loshkarev N, Dadinova L, Timofeeva T, Abramchuk S, Moiseenko A, Baratova L, Svergun D, Batishchev O, Membranes 11(10):772 (2021) DOI

SASDMJ5 – Four-component synthetic liposomes composed of 20% bPS + 13.3% POPC + 33.3% SM + 33.3% Chol lipid mixtures loaded with M1 protein

Four-component synthetic liposomes composed of 20% bPS + 13.3% POPC + 33.3% SM + 33.3% Chol lipid mixtures loaded with M1 protein (lipid:M1 molar ration 20:1)
MWexperimental 2000 kDa
MWexpected 2000 kDa
log I(s) 5.00×10-1 5.00×10-2 5.00×10-3 5.00×10-4
Four-component synthetic liposomes composed of 20% bPS + 13.3% POPC + 33.3% SM + 33.3% Chol lipid mixtures loaded with M1 protein (lipid:M1 molar ration 20:1) small angle scattering data  s, nm-1
(sRg)2I(s)/I(0)
Four-component synthetic liposomes composed of 20% bPS + 13.3% POPC + 33.3% SM + 33.3% Chol lipid mixtures loaded with M1 protein (lipid:M1 molar ration 20:1) Kratky plot 1.104 0 3 sRg

Data validation


Fits and models


log I(s)
 s, nm-1
Four-component synthetic liposomes composed of 20% bPS + 13.3% POPC + 33.3% SM + 33.3% Chol lipid mixtures loaded with M1 protein (lipid:M1 molar ration 20:1) OTHER [STATIC IMAGE] model

Synchrotron SAXS data from solutions of Four-component synthetic liposomes composed of 20% bPS + 13.3% POPC + 33.3% SM + 33.3% Chol lipid mixtures loaded with M1 protein in 100 mM NaCl, 50 mM MES buffer, pH 6.8 were collected on the EMBL P12 beam line at the PETRA III storage ring (DESY; Hamburg, Germany) using a Pilatus 6M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.124 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 1.00 mg/ml was measured at 10°C. 20 successive 0.050 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

NOTES: bPS = bovine brain extract of phosphatidylserines; POPC = 1-palmitoyl-2-oleoyl-sn- glycero-3-phosphatidylcholine; SM = egg sphingomyelin; Chol = cholesterol. The M1 matrix protein was isolated from purified influenza A/Puerto Rico/8/34(H1N1) virion. The image displays the restored electron density profile of the lipid bilayer. The additional zip file located in the full entry archive contains the SAXS multiple data set of synthetic liposomes composed of 20% bPS + 13.3% POPC + 33.3% SM + 33.3% Chol lipid mixtures loaded with M1 protein. Experimental small-angle scattering data are the files with extensions (*.dat). The best fits of the SAXS curves obtained by BILMIX program are the files with extenstions (*.fit). The restored electron density profiles of the lipid bilayer before and after loading with M1. The molar lipid/M1 ratios are the following: (1) no M1, (2) 67:1, (3) 30:1, (4) 20:1, (5) 15:1, (6) 10:1, (7) 7.5:1, (8) 5:1.

Tags: softmatter
Four-component synthetic liposomes composed of 20% bPS + 13.3% POPC + 33.3% SM + 33.3% Chol lipid mixtures loaded with M1 protein (lipid:M1 molar ration 20:1) (Comp4)
Mol. type   Other
Olig. state   Monomer
Mon. MW   2000 kDa
Chemical formula