| MWI(0) | 10 | kDa |
| MWexpected | 8 | kDa |
| VPorod | 27 | nm3 |
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log I(s)
9.97×100
9.97×10-1
9.97×10-2
9.97×10-3
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s, nm-1
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Conformational Disorder Analysis of the Conditionally Disordered Protein CP12 from Arabidopsis thaliana in Its Different Redox States
Del Giudice A, Gurrieri L, Galantini L, Fanti S, Trost P, Sparla F, Fermani S, International Journal of Molecular Sciences 24(11):9308 (2023) DOISASDSN2 – AtCP12-2 in a reduced form
CP12-2 (D77G)|
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Fits and models
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Rg, nm
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Synchrotron SAXS data from solutions of AtCP12-2 in a reduced form in 25 mM potassium phosphate, pH 7.5 were collected on the BM29 beam line at the ESRF (Grenoble, France) using a Pilatus 1M detector at a sample-detector distance of 2.9 m and at a wavelength of λ = 0.09919 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). Solute concentrations ranging between 0.3 and 5.6 mg/ml were measured at 4°C. 10 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted. The low angle data collected at lower concentration were merged with the highest concentration high angle data to yield the final composite scattering curve.
Tags:
idp
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