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Synchrotron SAXS data from solutions of Aspergillus niger beta-glucosidase (bgl1) in 50 mM sodium acetate, pH 5 were collected on the 16-ID (LiX) beamline at the National Synchrotron Light Source II (NSLS-II) storage ring (Upton, NY, USA) using a Pilatus3 X 1M, Pilatus3 X 900K detector at a sample-detector distance of 3.6 m and at a wavelength of λ = 0.08189 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 99.00 μl sample at 4.5 mg/ml was injected at a 0.35 ml/min flow rate onto a Biozen 3 µm dSEC-2, 200 Å column. 10 successive 2-second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
For the experiment, SAXS and WAXS detectors were used simultaneously. The detector positions are Sample-to-detector distance (in meters): SAXS - 3.562, WAXS - 0.317
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s, nm-1
