The Subnanometer Resolution Structure of the Glutamate Synthase 1.2-MDa Hexamer by Cryoelectron Microscopy and Its Oligomerization Behavior in Solution

Cottevieille M, Larquet E, Jonic S, Petoukhov M, Caprini G, Paravisi S, Svergun D, Vanoni M, Boisset N
Journal of Biological Chemistry 283(13):8237-8249 (2008 Mar)

doi: 10.1074/jbc.M708529200 		Submitted to SASBDB: 2021 May 31
Published in SASBDB:

SASDLZ6 – Hexamer - monomer equilibrium of Glutamate Synthase

Glutamate synthase [NADPH] large chainGlutamate synthase [NADPH] small chain experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Glutamate synthase [NADPH] large chain hexamer, 968 kDa Azospirillum brasilense protein
Glutamate synthase [NADPH] small chain hexamer, 296 kDa Azospirillum brasilense protein
Buffer: 25 mM Hepes/KOH, 1 mM EDTA, 1 mM dithiothreitol, pH: 7.5
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2007 Feb 19
RgGuinier 7.7 nm
Dmax 22.4 nm