Structural and functional insights into peptidoglycan access for the lytic amidase LytA of Streptococcus pneumoniae.

Mellroth P, Sandalova T, Kikhney A, Vilaplana F, Hesek D, Lee M, Mobashery S, Normark S, Svergun D, Henriques-Normark B, Achour A
MBio 5(1):e01120-13 (2014 Feb 11)

PMID: 24520066 doi: 10.1128/mBio.01120-13

Submitted to SASBDB: 2014 Feb 11 Published in SASBDB: 2016 Mar 16

SASDBF4 – Wild-type LytA (N-His6) with choline Sample: Lytic Amidase with choline dimer, 75 kDa Streptococcus pneumoniae protein Buffer: 20 mM Tris 150 mM NaCl 5 mM choline chloride 1 µM ZnCl2, pH: 7.5 Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2011 Jul 2 RgGuinier 4.9 nm Dmax 15.0 nm VolumePorod 115 nm3

SASDBG4 – Prophage LytA with choline Sample: Prophage Lytic Amidase with choline dimer, 73 kDa Streptococcus pneumoniae protein Buffer: 20 mM Tris 150 mM NaCl 5 mM choline chloride 1 µM ZnCl2, pH: 7.5 Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2011 Jul 2 RgGuinier 5.9 nm Dmax 17.0 nm VolumePorod 130 nm3

SASDBH4 – Wild-type LytA (N-His6) without choline Sample: Lytic Amidase without choline monomer, 37 kDa Streptococcus pneumoniae protein Buffer: 20 mM Tris 150 mM NaCl 1 µM ZnCl2, pH: 7.5 Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2011 Jul 3 RgGuinier 3.4 nm Dmax 12.5 nm VolumePorod 56 nm3

SASDBJ4 – Wild-type LytA choline-binding domain Sample: Lytic Amidase choline-binding domain dimer, 17 kDa Streptococcus pneumoniae protein Buffer: 20 mM Tris 150 mM NaCl 5 mM choline chloride 1 µM ZnCl2, pH: 7.5 Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2011 Dec 11 RgGuinier 3.3 nm Dmax 10.0 nm VolumePorod 49 nm3