From isolated polyelectrolytes to star-like assemblies: the role of sequence heterogeneity on the statistical structure of the intrinsically disordered neurofilament-low tail domain.

Kravikass M, Koren G, Saleh OA, Beck R
Eur Phys J E Soft Matter 47(2):13 (2024 Feb 15)

PMID: 38358563 doi: 10.1140/epje/s10189-024-00409-8

Submitted to SASBDB: 2023 Nov 30 Published in SASBDB: 2024 Jan 22

SASDT76 – Neurofilament low intrinsically disordered tail domain aggregate - 2.68 mg/ml - 20 mM Tris pH 8.0 Sample: Neurofilament light polypeptide (T445N; C-terminus, amino acids 399-553), 16 kDa Mus musculus protein Buffer: 20 mM Tris, pH: 8 Experiment: SAXS data collected at EMBL P12, PETRA III on 2023 Sep 7 RgGuinier 26.4 nm

SASDT86 – Neurofilament low intrinsically disordered tail domain aggregate - 1.80 mg/ml - 20 mM Tris pH 8.0 Sample: Neurofilament light polypeptide (T445N; C-terminus, amino acids 399-553), 16 kDa Mus musculus protein Buffer: 20 mM Tris, pH: 8 Experiment: SAXS data collected at EMBL P12, PETRA III on 2023 Sep 7 RgGuinier 21.9 nm

SASDT96 – Neurofilament low intrinsically disordered tail domain aggregate - 1.00 mg/ml - 20 mM Tris pH 8.0 Sample: Neurofilament light polypeptide (T445N; C-terminus, amino acids 399-553), 16 kDa Mus musculus protein Buffer: 20 mM Tris, pH: 8 Experiment: SAXS data collected at EMBL P12, PETRA III on 2023 Sep 7 RgGuinier 22.9 nm

SASDTA6 – Neurofilament low intrinsically disordered tail domain aggregate - 0.50 mg/ml - 20 mM Tris pH 8.0 Sample: Neurofilament light polypeptide (T445N; C-terminus, amino acids 399-553), 16 kDa Mus musculus protein Buffer: 20 mM Tris, pH: 8 Experiment: SAXS data collected at EMBL P12, PETRA III on 2023 Sep 7 RgGuinier 22.6 nm

SASDTB6 – Neurofilament low intrinsically disordered tail domain aggregate - 1.3 mg/ml - 20 mM Tris pH 8.0, 150 mM NaCl Sample: Neurofilament light polypeptide (T445N; C-terminus, amino acids 399-553), 16 kDa Mus musculus protein Buffer: 20 mM Tris pH 8.00, 150 mM NaCl, pH: Experiment: SAXS data collected at EMBL P12, PETRA III on 2023 Sep 7 RgGuinier 17.8 nm

SASDTC6 – Neurofilament low intrinsically disordered tail domain aggregate - 0.73 mg/ml - 20 mM Tris pH 8.0, 150 mM NaCl Sample: Neurofilament light polypeptide (T445N; C-terminus, amino acids 399-553), 16 kDa Mus musculus protein Buffer: 20 mM Tris pH 8.00, 150 mM NaCl, pH: Experiment: SAXS data collected at EMBL P12, PETRA III on 2023 Sep 7 RgGuinier 19.4 nm

SASDTD6 – Neurofilament low intrinsically disordered tail domain aggregate - 0.57 mg/ml - 20 mM Tris pH 8.0, 150 mM NaCl Sample: Neurofilament light polypeptide (T445N; C-terminus, amino acids 399-553), 16 kDa Mus musculus protein Buffer: 20 mM Tris pH 8.00, 150 mM NaCl, pH: Experiment: SAXS data collected at EMBL P12, PETRA III on 2023 Sep 7 RgGuinier 22.2 nm

SASDTE6 – Neurofilament low intrinsically disordered tail domain aggregate - 0.24 mg/ml - 20 mM Tris pH 8.0, 150 mM NaCl Sample: Neurofilament light polypeptide (T445N; C-terminus, amino acids 399-553), 16 kDa Mus musculus protein Buffer: 20 mM Tris pH 8.00, 150 mM NaCl, pH: Experiment: SAXS data collected at EMBL P12, PETRA III on 2023 Sep 7 RgGuinier 21.2 nm

SASDTF6 – Neurofilament low intrinsically disordered tail domain aggregate - 2.00 mg/ml - 20 mM Tris pH 8.0, 250 mM NaCl Sample: Neurofilament light polypeptide (T445N; C-terminus, amino acids 399-553), 16 kDa Mus musculus protein Buffer: 20 mM Tris pH 8.00, 250 mM NaCl, pH: Experiment: SAXS data collected at EMBL P12, PETRA III on 2023 Sep 7 RgGuinier 16.7 nm

SASDTG6 – Neurofilament low intrinsically disordered tail domain aggregate - 1.5 mg/ml - 20 mM Tris pH 8.0, 250 mM NaCl Sample: Neurofilament light polypeptide (T445N; C-terminus, amino acids 399-553), 16 kDa Mus musculus protein Buffer: 20 mM Tris pH 8.00, 250 mM NaCl, pH: Experiment: SAXS data collected at EMBL P12, PETRA III on 2023 Sep 7 RgGuinier 20.4 nm

SASDTH6 – Neurofilament low intrinsically disordered tail domain aggregate - 0.69 mg/ml - 20 mM Tris pH 8.0, 250 mM NaCl Sample: Neurofilament light polypeptide (T445N; C-terminus, amino acids 399-553), 16 kDa Mus musculus protein Buffer: 20 mM Tris pH 8.00, 250 mM NaCl, pH: Experiment: SAXS data collected at EMBL P12, PETRA III on 2023 Sep 7 RgGuinier 29.9 nm

SASDTJ6 – Neurofilament low intrinsically disordered tail domain aggregate - 2.3 mg/ml - 20 mM Tris pH 8.0, 350 mM NaCl Sample: Neurofilament light polypeptide (T445N; C-terminus, amino acids 399-553), 16 kDa Mus musculus protein Buffer: 20 mM Tris pH 8.00, 350 mM NaCl, pH: Experiment: SAXS data collected at EMBL P12, PETRA III on 2023 Sep 7 RgGuinier 17.7 nm

SASDTK6 – Neurofilament low intrinsically disordered tail domain aggregate - 1.5 mg/ml - 20 mM Tris pH 8.0, 350 mM NaCl Sample: Neurofilament light polypeptide (T445N; C-terminus, amino acids 399-553), 16 kDa Mus musculus protein Buffer: 20 mM Tris pH 8.00, 350 mM NaCl, pH: Experiment: SAXS data collected at EMBL P12, PETRA III on 2023 Sep 7 RgGuinier 17.4 nm

SASDTL6 – Neurofilament low intrinsically disordered tail domain aggregate - 0.96 mg/ml - 20 mM Tris pH 8.0, 350 mM NaCl Sample: Neurofilament light polypeptide (T445N; C-terminus, amino acids 399-553), 16 kDa Mus musculus protein Buffer: 20 mM Tris pH 8.00, 350 mM NaCl, pH: Experiment: SAXS data collected at EMBL P12, PETRA III on 2023 Sep 7 RgGuinier 17.8 nm

SASDTM6 – Neurofilament low intrinsically disordered tail domain aggregate - 0.6 mg/ml - 20 mM Tris pH 8.0, 350 mM NaCl Sample: Neurofilament light polypeptide (T445N; C-terminus, amino acids 399-553), 16 kDa Mus musculus protein Buffer: 20 mM Tris pH 8.00, 350 mM NaCl, pH: Experiment: SAXS data collected at EMBL P12, PETRA III on 2023 Sep 7 RgGuinier 22.4 nm

SASDTN6 – Neurofilament low intrinsically disordered tail domain aggregate - 2.5 mg/ml - 20 mM Tris pH 8.0, 500 mM NaCl Sample: Neurofilament light polypeptide (T445N; C-terminus, amino acids 399-553), 16 kDa Mus musculus protein Buffer: 20 mM Tris pH 8.00, 500 mM NaCl, pH: Experiment: SAXS data collected at EMBL P12, PETRA III on 2023 Sep 7 RgGuinier 20.1 nm

SASDTP6 – Neurofilament low intrinsically disordered tail domain aggregate - 1.19 mg/ml - 20 mM Tris pH 8.0, 500 mM NaCl Sample: Neurofilament light polypeptide (T445N; C-terminus, amino acids 399-553), 16 kDa Mus musculus protein Buffer: 20 mM Tris pH 8.00, 500 mM NaCl, pH: Experiment: SAXS data collected at EMBL P12, PETRA III on 2023 Sep 7 RgGuinier 17.2 nm

SASDTQ6 – Neurofilament low intrinsically disordered tail domain aggregate - 0.45 mg/ml - 20 mM Tris pH 8.0, 500 mM NaCl Sample: Neurofilament light polypeptide (T445N; C-terminus, amino acids 399-553), 16 kDa Mus musculus protein Buffer: 20 mM Tris pH 8.00, 500 mM NaCl, pH: Experiment: SAXS data collected at EMBL P12, PETRA III on 2023 Sep 7 RgGuinier 22.3 nm

SASDTR6 – Neurofilament low intrinsically disordered tail domain aggregate - 0.66 mg/ml - 20 mM Tris pH 8.0, 500 mM NaCl Sample: Neurofilament light polypeptide (T445N; C-terminus, amino acids 399-553), 16 kDa Mus musculus protein Buffer: 20 mM Tris pH 8.00, 500 mM NaCl, pH: Experiment: SAXS data collected at EMBL P12, PETRA III on 2023 Sep 7 RgGuinier 17.8 nm

SASDTS6 – Truncated Neurofilament low intrinsically disordered tail domain (amino acids 441-543) polyelectrolyte - 1.1 mg/ml - 20 mM Tris pH 8.0 Sample: Neurofilament light polypeptide (T445N; C-terminus, amino acids 441-543) monomer, 12 kDa Mus musculus protein Buffer: 20 mM Tris, pH: 8 Experiment: SAXS data collected at EMBL P12, PETRA III on 2023 Sep 7 RgGuinier 4.3 nm

SASDTT6 – Truncated Neurofilament low intrinsically disordered tail domain (amino acids 441-543) polyelectrolyte - 0.8 mg/ml - 20 mM Tris pH 8.0 Sample: Neurofilament light polypeptide (T445N; C-terminus, amino acids 441-543) monomer, 12 kDa Mus musculus protein Buffer: 20 mM Tris, pH: 8 Experiment: SAXS data collected at EMBL P12, PETRA III on 2023 Sep 7 RgGuinier 4.4 nm

SASDTU6 – Truncated Neurofilament low intrinsically disordered tail domain (amino acids 441-543) polyelectrolyte - 0.6 mg/ml - 20 mM Tris pH 8.0 Sample: Neurofilament light polypeptide (T445N; C-terminus, amino acids 441-543) monomer, 12 kDa Mus musculus protein Buffer: 20 mM Tris, pH: 8 Experiment: SAXS data collected at EMBL P12, PETRA III on 2023 Sep 7 RgGuinier 4.7 nm

SASDTV6 – Truncated Neurofilament low intrinsically disordered tail domain (amino acids 441-543) polyelectrolyte - 1 mg/ml - 20 mM Tris pH 8.0, 50 mM NaCl Sample: Neurofilament light polypeptide (T445N; C-terminus, amino acids 441-543) monomer, 12 kDa Mus musculus protein Buffer: 20 mM Tris pH 8.00, 50 mM NaCl, pH: Experiment: SAXS data collected at EMBL P12, PETRA III on 2023 Sep 7 RgGuinier 4.2 nm

SASDTW6 – Truncated Neurofilament low intrinsically disordered tail domain (amino acids 441-543) polyelectrolyte - 0.5 mg/ml - 20 mM Tris pH 8.0, 50 mM NaCl Sample: Neurofilament light polypeptide (T445N; C-terminus, amino acids 441-543) monomer, 12 kDa Mus musculus protein Buffer: 20 mM Tris pH 8.00, 50 mM NaCl, pH: Experiment: SAXS data collected at EMBL P12, PETRA III on 2023 Sep 7 RgGuinier 4.6 nm

SASDTX6 – Truncated Neurofilament low intrinsically disordered tail domain (amino acids 441-543) polyelectrolyte - 0.3 mg/ml - 20 mM Tris pH 8.0, 50 mM NaCl Sample: Neurofilament light polypeptide (T445N; C-terminus, amino acids 441-543) monomer, 12 kDa Mus musculus protein Buffer: 20 mM Tris pH 8.00, 50 mM NaCl, pH: Experiment: SAXS data collected at EMBL P12, PETRA III on 2023 Sep 7 RgGuinier 4.2 nm

SASDTY6 – Truncated Neurofilament low intrinsically disordered tail domain (amino acids 441-543) polyelectrolyte - 1.5 mg/ml - 20 mM Tris pH 8.0, 150 mM NaCl Sample: Neurofilament light polypeptide (T445N; C-terminus, amino acids 441-543) monomer, 12 kDa Mus musculus protein Buffer: 20 mM Tris pH 8.00, 150 mM NaCl, pH: Experiment: SAXS data collected at EMBL P12, PETRA III on 2023 Sep 7 RgGuinier 4.3 nm

SASDTZ6 – Truncated Neurofilament low intrinsically disordered tail domain (amino acids 441-543) polyelectrolyte - 0.8 mg/ml - 20 mM Tris pH 8.0, 150 mM NaCl Sample: Neurofilament light polypeptide (T445N; C-terminus, amino acids 441-543) monomer, 12 kDa Mus musculus protein Buffer: 20 mM Tris pH 8.00, 150 mM NaCl, pH: Experiment: SAXS data collected at EMBL P12, PETRA III on 2023 Sep 7 RgGuinier 4.7 nm

SASDT27 – Truncated Neurofilament low intrinsically disordered tail domain (amino acids 441-543) polyelectrolyte - 0.3 mg/ml - 20 mM Tris pH 8.0, 150 mM NaCl Sample: Neurofilament light polypeptide (T445N; C-terminus, amino acids 441-543) monomer, 12 kDa Mus musculus protein Buffer: 20 mM Tris pH 8.00, 150 mM NaCl, pH: Experiment: SAXS data collected at EMBL P12, PETRA III on 2023 Sep 7 RgGuinier 4.5 nm

SASDT37 – Truncated Neurofilament low intrinsically disordered tail domain (amino acids 441-543) polyelectrolyte - 1 mg/ml - 20 mM Tris pH 8.0, 250 mM NaCl Sample: Neurofilament light polypeptide (T445N; C-terminus, amino acids 441-543) monomer, 12 kDa Mus musculus protein Buffer: 20 mM Tris pH 8.00, 250 mM NaCl, pH: Experiment: SAXS data collected at EMBL P12, PETRA III on 2023 Sep 7 RgGuinier 4.6 nm

SASDT47 – Truncated Neurofilament low intrinsically disordered tail domain (amino acids 441-543) polyelectrolyte - 0.5 mg/ml - 20 mM Tris pH 8.0, 250 mM NaCl Sample: Neurofilament light polypeptide (T445N; C-terminus, amino acids 441-543) monomer, 12 kDa Mus musculus protein Buffer: 20 mM Tris pH 8.00, 250 mM NaCl, pH: Experiment: SAXS data collected at EMBL P12, PETRA III on 2023 Sep 7 RgGuinier 4.6 nm

SASDT57 – Truncated Neurofilament low intrinsically disordered tail domain (amino acids 441-543) polyelectrolyte - 0.3 mg/ml - 20 mM Tris pH 8.0, 250 mM NaCl Sample: Neurofilament light polypeptide (T445N; C-terminus, amino acids 441-543) monomer, 12 kDa Mus musculus protein Buffer: 20 mM Tris pH 8.00, 250 mM NaCl, pH: Experiment: SAXS data collected at EMBL P12, PETRA III on 2023 Sep 7 RgGuinier 4.4 nm

SASDT67 – Truncated Neurofilament low intrinsically disordered tail domain (amino acids 441-543) polyelectrolyte - 1.5 mg/ml - 20 mM Tris pH 8.0, 500 mM NaCl Sample: Neurofilament light polypeptide (T445N; C-terminus, amino acids 441-543) monomer, 12 kDa Mus musculus protein Buffer: 20 mM Tris pH 8.00, 500 mM NaCl, pH: Experiment: SAXS data collected at B21, Diamond Light Source on 2023 Sep 7 RgGuinier 4.0 nm

SASDT77 – Truncated Neurofilament low intrinsically disordered tail domain (amino acids 441-543) polyelectrolyte - 0.78 mg/ml - 20 mM Tris pH 8.0, 500 mM NaCl Sample: Neurofilament light polypeptide (T445N; C-terminus, amino acids 441-543) monomer, 12 kDa Mus musculus protein Buffer: 20 mM Tris pH 8.00, 500 mM NaCl, pH: Experiment: SAXS data collected at B21, Diamond Light Source on 2023 Sep 7 RgGuinier 4.0 nm

SASDT87 – Truncated Neurofilament low intrinsically disordered tail domain (amino acids 441-543) polyelectrolyte - 0.38 mg/ml - 20 mM Tris pH 8.0, 500 mM NaCl Sample: Neurofilament light polypeptide (T445N; C-terminus, amino acids 441-543) monomer, 12 kDa Mus musculus protein Buffer: 20 mM Tris pH 8.00, 500 mM NaCl, pH: Experiment: SAXS data collected at B21, Diamond Light Source on 2023 Sep 7 RgGuinier 4.2 nm