Functionally specific binding regions of microtubule-associated protein 2c exhibit distinct conformations and dynamics.

Melková K, Zapletal V, Jansen S, Nomilner E, Zachrdla M, Hritz J, Nováček J, Zweckstetter M, Jensen MR, Blackledge M, Žídek L
J Biol Chem 293(34):13297-13309 (2018 Aug 24)

PMID: 29925592 doi: 10.1074/jbc.RA118.001769

Submitted to SASBDB: 2017 Dec 21 Published in SASBDB: 2018 Jun 19

SASDD62 – Microtubule associated protein MAP2c (isoform 3); 12 mg/ml Sample: Microtubule-associated protein 2, isoform 3 monomer, 49 kDa Rattus norvegicus protein Buffer: 50 mM MOPS, 150 mM NaCl, 0.03% NaN3, pH: 6.9 Experiment: SAXS data collected at BM29, ESRF on 2017 Apr 21 RgGuinier 8.3 nm

SASDD72 – Microtubule associated protein MAP2c (isoform 3); 6 mg/ml Sample: Microtubule-associated protein 2, isoform 3 monomer, 49 kDa Rattus norvegicus protein Buffer: 50 mM MOPS, 150 mM NaCl, 0.03% NaN3, pH: 6.9 Experiment: SAXS data collected at BM29, ESRF on 2017 Apr 21 RgGuinier 7.9 nm

SASDD82 – Microtubule associated protein MAP2c (isoform 3); 3 mg/ml Sample: Microtubule-associated protein 2, isoform 3 monomer, 49 kDa Rattus norvegicus protein Buffer: 50 mM MOPS, 150 mM NaCl, 0.03% NaN3, pH: 6.9 Experiment: SAXS data collected at BM29, ESRF on 2017 Apr 21 RgGuinier 6.9 nm

SASDD92 – Microtubule associated protein MAP2c (isoform 3); 1.5 mg/ml Sample: Microtubule-associated protein 2, isoform 3 monomer, 49 kDa Rattus norvegicus protein Buffer: 50 mM MOPS, 150 mM NaCl, 0.03% NaN3, pH: 6.9 Experiment: SAXS data collected at BM29, ESRF on 2017 Apr 21 RgGuinier 6.7 nm

SASDDA2 – Phosphorylated Microtubule associated protein MAP2c (isoform 3); 13.6 mg/ml Sample: Microtubule-associated protein 2, isoform 3 monomer, 49 kDa Rattus norvegicus protein Buffer: 50 mM MOPS, 150 mM NaCl, 0.03% NaN3, pH: 6.9 Experiment: SAXS data collected at BM29, ESRF on 2017 Apr 21 RgGuinier 7.9 nm

SASDDB2 – Phosphorylated Microtubule associated protein MAP2c (isoform 3); 6.8 mg/ml Sample: Microtubule-associated protein 2, isoform 3 monomer, 49 kDa Rattus norvegicus protein Buffer: 50 mM MOPS, 150 mM NaCl, 0.03% NaN3, pH: 6.9 Experiment: SAXS data collected at BM29, ESRF on 2017 Apr 21 RgGuinier 7.3 nm

SASDDC2 – Phosphorylated Microtubule associated protein MAP2c (isoform 3); 1.7 mg/ml Sample: Microtubule-associated protein 2, isoform 3 monomer, 49 kDa Rattus norvegicus protein Buffer: MOPS map2c buffer, pH: 6.9 Experiment: SAXS data collected at BM29, ESRF on 2017 Apr 21 RgGuinier 6.7 nm