Did evolution create a flexible ligand-binding cavity in the urokinase receptor through deletion of a plesiotypic disulfide bond?

Leth JM, Mertens HDT, Leth-Espensen KZ, Jørgensen TJD, Ploug M
J Biol Chem (2019 Mar 20)

PMID: 30894413 doi: 10.1074/jbc.RA119.007847

Submitted to SASBDB: 2019 Jan 30 Published in SASBDB: 2019 Mar 20

SASDF82 – Urokinase plasminogen activator surface receptor, uPAR H47C-N259C, complex with urokinase-type plasminogen activator (Amino Terminal Fragment, ATF). Sample: Urokinase plasminogen activator surface receptor monomer, 37 kDa Homo sapiens protein Urokinase-type plasminogen activator (Amino Terminal Fragment) monomer, 16 kDa Homo sapiens protein Buffer: 20 mM PBS, 5 %(v/v) glycerol, 50 mM NaSO4,, pH: 7.4 Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2011 Jun 18 RgGuinier 2.6 nm Dmax 8.2 nm VolumePorod 102 nm3

SASDF92 – Urokinase plasminogen activator surface receptor, uPAR, T51C-V70C Sample: Urokinase plasminogen activator surface receptor monomer, 37 kDa Homo sapiens protein Buffer: 20 mM PBS, 5 %(v/v) glycerol, pH: 7.4 Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 Dec 1 RgGuinier 2.5 nm Dmax 8.9 nm VolumePorod 55 nm3

SASDFA2 – Urokinase plasminogen activator surface receptor, uPAR T51C-V70C, complex with urokinase-type plasminogen activator (Amino Terminal Fragment, ATF). Sample: Urokinase plasminogen activator surface receptor monomer, 37 kDa Homo sapiens protein Urokinase-type plasminogen activator (Amino Terminal Fragment) monomer, 16 kDa Homo sapiens protein Buffer: 20 mM PBS, 5 %(v/v) glycerol, pH: 7.4 Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 Dec 1 RgGuinier 2.6 nm Dmax 8.5 nm VolumePorod 77 nm3

SASDFB2 – Urokinase plasminogen activator surface receptor, uPAR, K50C-V70C Sample: Urokinase plasminogen activator surface receptor monomer, 37 kDa Homo sapiens protein Buffer: 20 mM PBS, 5 %(v/v) glycerol, pH: 7.4 Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 May 5 RgGuinier 2.5 nm Dmax 9.3 nm VolumePorod 66 nm3

SASDFC2 – Urokinase plasminogen activator surface receptor, uPAR K50C-V70C, complex with urokinase-type plasminogen activator (Amino Terminal Fragment, ATF). Sample: Urokinase plasminogen activator surface receptor monomer, 37 kDa Homo sapiens protein Urokinase-type plasminogen activator (Amino Terminal Fragment) monomer, 16 kDa Homo sapiens protein Buffer: 20 mM PBS, 5 %(v/v) glycerol, pH: 7.4 Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 May 5 RgGuinier 2.6 nm Dmax 9.0 nm VolumePorod 66 nm3