Immunosuppressive Yersinia Effector YopM Binds DEAD Box Helicase DDX3 to Control Ribosomal S6 Kinase in the Nucleus of Host Cells.

Berneking L, Schnapp M, Rumm A, Trasak C, Ruckdeschel K, Alawi M, Grundhoff A, Kikhney AG, Koch-Nolte F, Buck F, Perbandt M, Betzel C, Svergun DI, Hentschke M, Aepfelbacher M
PLoS Pathog 12(6):e1005660 (2016 Jun)

PMID: 27300509 doi: 10.1371/journal.ppat.1005660

Submitted to SASBDB: 2015 Jul 6 Published in SASBDB: 2016 Jun 14

SASDAU8 – Immunosuppressive virulence protein YopM Sample: Yersinia outer protein M (34-481) dimer, 101 kDa Yersinia enterocolitica protein Buffer: 50 mM HEPES 150 mM NaCl, pH: 7.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Nov 13 RgGuinier 3.9 nm Dmax 11.5 nm VolumePorod 154 nm3

SASDAV8 – DEAD box helicase DDX3 Sample: DEAD box RNA helicase DDX3 (51-418) monomer, 42 kDa Yersinia enterocolitica protein Buffer: 50 mM HEPES 150 mM NaCl, pH: 7.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Nov 13 RgGuinier 2.8 nm Dmax 12.0 nm VolumePorod 80 nm3

SASDAW8 – 2:1 YopM:DDX3 complex Sample: Yersinia outer protein M (34-481) dimer, 101 kDa Yersinia enterocolitica protein DEAD box RNA helicase DDX3 (51-418) monomer, 42 kDa Yersinia enterocolitica protein Buffer: 50 mM HEPES 150 mM NaCl, pH: 7.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Jun 14 RgGuinier 4.0 nm Dmax 15.0 nm VolumePorod 190 nm3