The solution structures of higher-order human telomere G-quadruplex multimers.

Monsen RC, Chakravarthy S, Dean WL, Chaires JB, Trent JO
Nucleic Acids Res (2021 Jan 19)

PMID: 33469644 doi: 10.1093/nar/gkaa1285

Submitted to SASBDB: 2020 Nov 9 Published in SASBDB: 2020 Nov 23

SASDKF3 – Human telomere monomer G4 hybrid-2 form (2JSL) Sample: Human telomere G-quadruplex hybrid-2 form monomer, 8 kDa synthetic construct DNA Buffer: 6 mM Na2HPO4, 2 mM NaH2PO4, 1 mM Na2EDTA, 185 mM KCl, pH: 7.2 Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2019 Aug 14 RgGuinier 1.2 nm Dmax 3.8 nm VolumePorod 9 nm3

SASDKG3 – Human wild-type telomere 48-mer d(TTAGGG)8 Sample: Human Telomere Repeat (TTAGGG)8 monomer, 15 kDa synthetic construct DNA Buffer: 6 mM Na2HPO4, 2 mM NaH2PO4, 1 mM Na2EDTA, 185 mM KCl, pH: 7.2 Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2019 Aug 14 RgGuinier 1.9 nm Dmax 6.4 nm VolumePorod 16 nm3

SASDKH3 – Human wild-type telomere 72mer d(TTAGGG)12 Sample: Human Telomere Repeat (TTAGGG)12 monomer, 23 kDa synthetic construct DNA Buffer: 6 mM Na2HPO4, 2 mM NaH2PO4, 1 mM Na2EDTA, 185 mM KCl, pH: 7.2 Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2019 Aug 14 RgGuinier 2.5 nm Dmax 8.7 nm VolumePorod 26 nm3

SASDKJ3 – Human telomere DNA d(TTAGGG)96 Sample: Human Telomere 96mer monomer, 31 kDa synthetic construct DNA Buffer: 6 mM Na2HPO4, 2 mM NaH2PO4, 1 mM Na2EDTA, 185 mM KCl, pH: 7.2 Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2020 Feb 19 RgGuinier 3.2 nm Dmax 10.9 nm VolumePorod 38 nm3